Summary
Aims
The study reports the feasibility and efficiency of vascular endothelial growth factor (VEGF) delivery using nanoparticles synthesized from glycidyl methacrylated dextran (Dex‐GMA) and gelatin for therapeutic angiogenesis.
Methods
The nanoparticles were prepared using phase separation method, and the drug release profile was determined by ELISA study. The bioactivity of VEGF‐incorporated nanoparticles (VEGF‐NPs) were determined using tube formation assay. A rabbit hind limb ischemia model was employed to evaluate the in vivo therapeutic effect. Blood perfusion was measured by single‐photon emission computed tomography (SPECT). Vessel formation was evaluated by contrast angiography and immunohistochemistry.
Results
The nanoparticles synthesized were spherical in shape with evenly distributed size of about 130 ± 3.5 nm. The VEGF encapsulated was released in a biphase manner, with the majority of 69% released over 1–12 days. Tube formation assays showed increased tubular structures by VEGF‐NP compared with empty nanoparticles and no treatment. Both free VEGF and VEGF‐NP significantly increased blood perfusion compared with empty nanoparticles (both P < 0.001), but it was much higher in VEGF‐NP‐treated limbs (P < 0.001). Contrast angiography and immunohistological analysis also revealed more significant collateral artery formation and higher capillary density in VEGF‐NP‐treated limbs.
Conclusions
Dex‐GMA and gelatin‐based nanoparticles could provide sustained release of VEGF and may serve as a new way for angiogenesis.
IT can significantly attenuate the systemic inflammatory response and improve a damaged cardiac function, but it does not reduce the in-hospital mortality rate.
Acellularized PAVs processed by modified detergent-enzymatic acellularization procedure can be used for cardiovascular tissue-engineered grafts as matrix scaffolds without risk of PERV transmission.
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