The Raman probe plays an essential role in sensitive surfaceenhanced Raman scattering (SERS) assay. Here, a novel Raman probe was developed by assembling gold nanoparticles in triangular pyramid DNA (TP-Au NPs). Such probe with intense electromagnetic hot spots can provide dramatically enhanced Raman scattering. Through assembling recognition DNA on one corner of the TP-DNA, the recognition event is definite and designable. The probe was characterized through TEM, and its SERS superiority was investigated. As models, circulating tumor cells and exosomes were detected with high sensitivity and selectivity by using this probe. Meanwhile, the developed SERS probe can also perform well in real world samples.
Cancer
theranostics is of great significance in the personalized
therapy. In this work, stable Janus nanoparticles (JNPs) containing
PEG and two kinds of DNAs were prepared by means of “click
chemistry”. In response to ATP or acid condition, the prepared
JNPs could form Au NP dimers, which facilitate in situ SERS detection
and SERS imaging analysis of cancer cells due to the formation of
“hot spots” in the nanogap between the Au NP dimers.
A detection limit of 2.3 × 10–9 M was obtained
for ATP. As for a pH sensor, the SERS signals increased with the decrease
of pH value from 8.0 to 4.0. In situ monitoring of ATP or acid condition
in cancer cells by SERS can improve the accuracy and sensitivity of
diagnosis. Moreover, drugs and photosensitizers loaded on the other
side of JNPs led to the chemotherapy/photodynamic therapy synergistic
antitumor effect, which was verified by in vitro and in vivo experiments.
Given the excellent performance in SERS detection and cancer therapy,
the developed JNPs hold considerable potential in cancer theranostics.
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