Carboxymethylated dextran (CMD)-coated magnetic iron oxide nanoparticles (MNPs) were synthesized using a co-precipitation method. Compared to neutral dextran coated MNPs, the CMD coating provides good dispersity and colloidal stability to the CMD-MNPs. In particular, the carboxyl groups on the CMD can be readily activated for covalent attachment of antibody molecules. The superparamagnetic property of the antibody-covered CMD-MNPs enables the captured antigen to be separated from the sample solution and CMD coating significantly reduces the nonspecific binding of the nanoparticles. Regeneration of the anti-BSA antibody-covered CMD-MNPs with NaOH does not significantly decrease the antibody activity, and the repeated magnetic separation and washing steps cause only small loss of the starting materials. The method was found to be highly reproducible (RSDs for BSA adsorption and desorption are between 0.78% and 5.1%). The anti-BSA antibody-covered CMD-MNPs possess good selectivity and are able to capture protein antigens from real samples.
Some enterprises of the Pearl River Delta had invested in Hunan province, and they played significant roles in Hunan’s economic development. However, the contribution to economic growth in general was not obvious in Hunan. According to the quantitative analysis that Hunan undertook an obstacle to the shift of the Pearl River Delta Industry. It could be found: The actual utilization of foreign investment in Hunan, Hunan road length, the balance of rural and urban residents in Hunan, Hunan consumption level and years of commodity market turnover, these five variables are the key barriers that Hunan could not take over the transfer successfully in Internet era. Five variables representing the Government's institutional arrangements for attracting foreign investment, transport infrastructure, subject to cultural constraints on investment entrepreneurial spirit, the regional market factors in Hunan that be decided from the regional market size and level of development. Therefore, to speed up the transfer of industries in Hunan docking the Pearl River Delta, it must be focused on several aspects of the above and then do the reform and innovation.
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