Significance Given the current pressing need to more fully understand the methane cycle on Earth, in particular, unidentified sinks for methane, identifying and quantifying novel sinks for methane is fundamental importance. Here, we provide previously unidentified direct evidence for the nitrite-dependent anaerobic methane oxidation (n-damo) process as a previously overlooked microbial methane sink in wetlands by stable isotope measurements, quantitative PCR assays, and 16S rRNA and particulate methane monooxygenase gene clone library analyses. It is estimated that n-damo could consume 4.1–6.1 Tg of CH 4 m −2 per year in wetlands under anaerobic conditions, which is roughly 2–6% of current worldwide CH 4 flux estimates for wetlands. Given the worldwide increase in nitrogen pollution, this methane sink may become more important in the future.
Oxalic acid secretion from roots is considered to be an important mechanism for aluminum (Al) resistance in buckwheat (Fygopyrum esculentum Moench). Nonetheless, only a single Al-resistant buckwheat cultivar was used to investigate the significance of oxalic acid in detoxifying Al. In this study, we investigated two buckwheat cultivars, Jiangxi (Al resistant) and Shanxi (Al sensitive), which showed significant variation in their resistance to Al stress. In the presence of 0 to 100 mM Al, the inhibition of root elongation was greater in Shanxi than that in Jiangxi, and the Al content of root apices (0-10 mm) was much lower in Jiangxi. However, the dependence of oxalic acid secretion on external Al concentration and the time course for secretion were similar in both cultivars. Furthermore, the variation in Al-induced oxalic acid efflux along the root was similar, showing a 10-fold greater efflux from the apical 0-to 5-mm region than from the 5-to 10-mm region. These results suggest that both Shanxi and Jiangxi possess an equal capacity for Al-dependent oxalic acid secretion. Another two potential Al resistance mechanisms, i.e. Al-induced alkalinization of rhizosphere pH and root inorganic phosphate release, were also not involved in their differential Al resistance. However, after longer treatments in Al (10 d), the concentrations of phosphorus and Al in the roots of the Al-resistant cultivar Jiangxi were significantly higher than those in Shanxi. Furthermore, more Al was localized in the cell walls of the resistant cultivar. All these results suggest that while Al-dependent oxalic acid secretion might contribute to the overall high resistance to Al stress of buckwheat, this response cannot explain the variation in tolerance between these two cultivars. We present evidence suggesting the greater Al resistance in buckwheat is further related to the immobilization and detoxification of Al by phosphorus in the root tissues.Ionic aluminum (Al) is highly toxic to plant growth and appears to interfere with a number of physiological and biochemical processes (Rengel, 1992;Kochian, 1995). However, species vary widely in their ability to resist the harmful effect of Al, and significant differences in Al resistance have even been reported between genotypes of the same species (Yang et al., 2005). Over the past few decades, concerted efforts have been made to understand the genetic and physiological basis of Al resistance in many different species. As proposed by Taylor (1991), Al resistance mechanisms can be grouped into two categories. One is based on excluding Al from the root cells, and the other relies on improving the resistance of plants to the Al ions once they enter the cytosol. Among the likely exclusion mechanisms, a role for organic acid efflux has been well documented in several species (Ma, 2000;Ryan et al., 2001;Kochian et al., 2004). Other potential exclusion mechanisms include increases in rhizospheric pH (Degenhardt et al., 1998), phosphate efflux (Pellet et al., 1996), the secretion of proteins to bind Al ...
Background: Mitsugumin 53 (MG53 or TRIM72), a striated muscle-specific E3 ligase, promotes ubiquitin-dependent degradation of the insulin receptor and insulin receptor substrate-1 and subsequently induces insulin resistance, resulting in metabolic syndrome and type 2 diabetes mellitus (T2DM). However, it is unknown how MG53 from muscle regulates systemic insulin response and energy metabolism. Increasing evidence demonstrates that muscle secretes proteins as myokines or cardiokines that regulate systemic metabolic processes. We hypothesize that MG53 may act as a myokine/cardiokine, contributing to interorgan regulation of insulin sensitivity and metabolic homeostasis. Methods: Using perfused rodent hearts or skeletal muscle, we investigated whether high glucose, high insulin, or their combination (conditions mimicking metabolic syndrome or T2DM) alters MG53 protein concentration in the perfusate. We also measured serum MG53 levels in rodents and humans in the presence or absence of metabolic diseases, particularly T2DM. The effects of circulating MG53 on multiorgan insulin response were evaluated by systemic delivery of recombinant MG53 protein to mice. Furthermore, the potential involvement of circulating MG53 in the pathogenesis of T2DM was assessed by neutralizing blood MG53 with monoclonal antibodies in diabetic db/db mice. Finally, to delineate the mechanism underlying the action of extracellular MG53 on insulin signaling, we analyzed the potential interaction of MG53 with extracellular domain of insulin receptor using coimmunoprecipitation and surface plasmon resonance assays. Results: Here, we demonstrate that MG53 is a glucose-sensitive myokine/cardiokine that governs the interorgan regulation of insulin sensitivity. First, high glucose or high insulin induces MG53 secretion from isolated rodent hearts and skeletal muscle. Second, hyperglycemia is accompanied by increased circulating MG53 in humans and rodents with diabetes mellitus. Third, systemic delivery of recombinant MG53 or cardiac-specific overexpression of MG53 causes systemic insulin resistance and metabolic syndrome in mice, whereas neutralizing circulating MG53 with monoclonal antibodies has therapeutic effects in T2DM db/db mice. Mechanistically, MG53 binds to the extracellular domain of the insulin receptor and acts as an allosteric blocker. Conclusions: Thus, MG53 has dual actions as a myokine/cardiokine and an E3 ligase, synergistically inhibiting the insulin signaling pathway. Targeting circulating MG53 opens a new therapeutic avenue for T2DM and its complications.
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