BackgroundIt is essential to subculture the cells once cultured cells reach confluence. For this, trypsin is frequently applied to dissociate adhesive cells from the substratum. However, due to the proteolytic activity of trypsin, cell surface proteins are often cleaved, which leads to dysregulation of the cell functions.MethodsIn this study, a triplicate 2D-DIGE strategy has been performed to monitor trypsin-induced proteome alterations. The differentially expressed spots were identified by MALDI-TOF MS and validated by immunoblotting.Results36 proteins are found to be differentially expressed in cells treated with trypsin, and proteins that are known to regulate cell metabolism, growth regulation, mitochondrial electron transportation and cell adhesion are down-regulated and proteins that regulate cell apoptosis are up-regulated after trypsin treatment. Further study shows that bcl-2 is down-regulated, p53 and p21 are both up-regulated after trypsinization.ConclusionsIn summary, this is the first report that uses the proteomic approach to thoroughly study trypsin-induced cell physiological changes and provides researchers in carrying out their experimental design.

Land use is one of the key factors affecting soil erosion in the Loess Plateau of China. This paper examines soil erosion under different land uses and land-use combinations using 137 Cs tracing in the Yangjuangou Catchment, a tributary of the Yan River in the Loess Plateau of Northern Shaanxi Province. The results show that the order of 137 Cs activity in different land uses decreases sequentially from mature forest to grass to young forest to orchard to terrace crop, indicating that the mature forests had the lowest erosion rates while the terraced cropland produced the highest erosion amount. The majority of 137 Cs is distributed in the top 0-10 cm of the soil layer. The 137 Cs activity in mature forest and grass soil is signifi cantly higher than in other land uses. Three land-use combinations on the hillslope were selected to study the relationship between land-use combination and soil erosion. The mixtures of 'grass (6 years old) + mature forest (25 years old) + grass (25 years old)' and 'grass (6 years old) + young forest (6 years old) + mature forest (25 years old) + grass (25 years old)' are better for soil erosion control, lowering soil erosion amount by 42% compared with a land-use combination of 'grass (6 years old) and shrub (6 years old)'. The results provide an important basis for optimizing land-use combinations to control soil erosion on slopes and may also result in important ecological benefi ts.

No abstract

No abstract