The advent of the DNA sequencing age has led to a revolution in biology. The rapid and cost‐effective generation of high‐quality sequence data has transformed many fields, including those focused on discovering species and surveying biodiversity, monitoring movement of biological materials, forensic biology, and disease diagnostics. There is a need to build capacity to generate useful sequence data in countries with limited historical access to laboratory resources, so that researchers can benefit from the advantages offered by these data. Commonly used molecular techniques such as DNA extraction, PCR, and DNA sequencing are within the reach of small laboratories in many countries, with the main obstacles to successful implementation being lack of funding and limited practical experience. Here we describe a successful approach that we developed to obtain DNA sequence data during a small DNA barcoding project in Indonesia.
The island of Sulawesi has been highlighted as a globally important conservation area, across a range of evaluation criteria. Collection rates on the Sulawesi Island are the lowest among in Indonesia area. Taxonomic study also has been limited, with most experts reporting large numbers of undescribed species. The research of Araceae diversity in Sulawesi is subject which in general has not been studied critically. The main purpose of this research is concerned to inventory the diversity of Araceae genera. The exploration was conducted in each location used exploring method. The Araceae genera in Silui Mountain and Uluisimbone forest consist of 30 numbers, 24 species and 14 genera, including into 3 sub families (Aroideae, Monsteroideae and Pothoideae). Mostly Araceae were found at humid location, the river flows. Schismatoglottis calyptrata Zoll. & Mor. and Aglaonema simplex Bl. are dominant species for terrestrial Araceae, where as Scindapsus spp. and Pothoidium spp. are dominant species for climbing Araceae.
During plant inventory work in the forested areas of Mt Mesehe and Mt Merbuk, Jembrana district, Bali, twenty orchid species were collected. Among the species collected, five were confirmed as new records for Bali. The five newly recorded species are Calanthe zollingeri Rchb.f., Plocoglottis plicata (Roxb.) Ormerod, Podochilus serpyllifolius (Blume) Lindl., Styloglossum speciosum (Blume) T.Yukawa & P.J.Cribb, and Vanilla albida Blume. Descriptions of taxa and images are provided.
Recent collections from Sulawesi allow an investigation of Amomum subgenus Botryamomum K. Schum. and its three species, Amomum chrysogynia K. Schum., A. echinosphaera K. Schum., and A. melichroum K. Schum. It is concluded that this subgenus contains a single species, Amomum melichroum K. Schum. Amomum subgenus Botryamomum and Amomum melichroum are lectotypifi ed and Schumann ' s classifi cation of them as Alpinia melichroa (K. Schum.) K. Schum. in Alpinia sect. Botryamomum (K. Schum.) K. Schum. is accepted. An amplifi ed description including fl owers and fruits is provided. Alpinia melichroa is narrowly distributed in southeastern Sulawesi; a conservation assessment is given.
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