Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.
Mariana BD, Arisah H, Yenni, Selvawajayanti M. 2018. Seedless fruit pummelo induced by Gamma Ray irradiation: Fruit morphological characters and stability evaluation. Biodiversitas 19: 656-661. Pummelo is one of native citrus crops found in South East Asia including Indonesia. It is well known due to its big size and distinct taste compared to other citrus fruits. However, it contains many seeds, which may reduce the market demand and customer preferences. Therefore, the objective of this study was to generate seedless citrus by mutation techniques using gamma-ray radiation. The mutant induction of Pamelo Nambangan using gamma-ray radiation was conducted in 2003 using bud wood as plant materials. The bud wood was then irradiated with the dosage of 20, 40, and 60 Gy. The buds then grafted to Japansche Citroen rootstocks and maintained properly until the fruits could be observed. After three years of selection based on performance and fruit evaluation on MV2 generation, a mutant plant derived from 20 Gy irradiation treatments were obtained with improved character on the number of seeds. Pamelo Nambangan has more than 40 seeds/fruit and the mutant has less than 10 seeds/fruit on average. In the seedless mutant, it was observed that the mutant has embryo sac abortion leading to seedless fruit and low pollen viability (7.7%). The mutant has been released in 2016 as a new seedless pummelo variety under the name of ‘Pamindo Agrihorti’.
Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.
ABSTRAK.Plantlet jeruk hasil perbanyakan embriogenesis somatik (ES) in vitro telah banyak dihasilkan. Meskipun demikian, pertumbuhan vegetatif dan generatif di lapang belum dievaluasi. Tujuan dari penelitian ini adalah untuk mengevaluasi kemampuan pertumbuhan vegetatif dan generatif tanaman jeruk hasil sambung dengan plantlet asal ES dibandingkan mata tempel asal BPMT. Penelitian dilakukan di Kebun Percobaan Tlekung, Balai Penelitian Tanaman Jeruk dan Buah Subtropika, mulai September 2013 sampai dengan Desember 2016. Materi penelitian adalah tanaman jeruk dengan batang atas asal (a) plantlet hasil regenerasi melalui ES tanpa bagian akarnya dan (b) mata tempel yang berasal dari Blok Penggandaan Mata Tempel (BPMT), yang masing-masing disambungkan dan ditempelkan dengan batang bawah Japansche Citroen (JC) berumur 8 bulan setelah transplanting. Tanaman hasil sambung berumur 1 tahun dipindah dan ditanam di lapang dengan jarak tanam rapat 1,5 m x 1,5 m. Pengamatan pertumbuhan dilakukan mulai umur 18 -42 bulan setelah transplanting (BST). Hasil penelitian menunjukkan bahwa tanaman jeruk dengan batang atas hasil ES dapat tumbuh, berkembang, dan berproduksi sama dengan tanaman dengan batang atas asal BPMT. Tinggi tanaman, diameter batang atas, dan diameter batang bawah pada tanaman ES tidak berbeda nyata dengan tanaman BPMT. Tanaman pada dua perlakuan mulai berbunga pada umur 18 BST, dengan jumlah bunga, buah, dan persentase fruitset yang tidak berbeda nyata antarkedua perlakuan, demikian juga pada pembungaan pada tahun berikutnya. Jumlah buah pada tahun ke-2 berbuah (September 2014) dan akhir pengamatan (September 2016) menunjukkan terjadi kenaikan sebesar 215,7% dan 176,1% pada masing-masing perlakuan ES dan BPMT, sedangkan pada tahun ke-4 pembuahan (2016), perlakuan tanaman jeruk hasil ES mempunyai jumlah buah/tanaman dan berat buah total/tanaman lebih banyak secara nyata dibandingkan asal BPMT. Sifat fisik dan kualitas buah (vit C, total keasaman, dan TPT) yang dihasilkan relatif sama. Tanaman jeruk siam Kintamani yang berasal dari plantlet hasil perbanyakan ES in vitro dan disambungkan dengan batang bawah JC dapat tumbuh, berkembang, dan berproduksi dengan normal di lapang.Kata kunci: Siam Kintamani (Citrus nobilis Lour.); BPMT; Sambung; Tempel; Embriogenesis somatik ABSTRACT. The plantlets derived from citrus somatic embryogenesis (SE) in vitro have been widely produced. However, their vegetative and generative growth in the field has not been evaluated. The aimed of this research was to evaluate the ability of vegetative and generative growth both of SE and Budwood Multiplication Block (BMB) derived citrus plants. The research was conducted in Tlekung Experimental Garden, Indonesian Citrus and Subtropical Fruit Research Institute, from September 2013 to December 2016. The citrus plants derived from (a) root-decapitated plantlets and (b) buds come from BMB that were grafted and budded, respectively on 8 months old JC rootstock. One-year old grafted and budded plants were planted at field using a dense spacing (1.5 ...
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