Outbreaks of Salmonellosis remain a major public health problem globally. This study determined the diversity and antibiotic resistance gene profile of Salmonella enterica serovars isolated from humans and food animals. Using standard methods, Salmonella spp. were isolated from fecal samples, profiled for antimicrobial susceptibility and resistance genes. Seventy-one Salmonella isolates were recovered from both humans and food animals comprising cattle, sheep, and chicken. Forty-four serovars were identified, with dominant Salmonella Budapest (31.8%). Rare serovars were present in chicken (
Aims: Salmonella infection remains a major public health concern worldwide, contributing the economic burden of both industrialized and developing countries through the costs associated with surveillance, prevention, and treatment of disease. This zoonosis has a harmful health and economic impact in terms of death, hospitalization, and destruction of livestock on farms. To adapts the means of control and prevention against this threat, the phenotypical characterization of Salmonella strains, both those recently identified and those which have been conserved for a long time, is necessary. So, the aim of this study was to check quality of the salmonella strains first stored in storage tubes in NRC of salmonella of Institute Pasteur of Côte d'Ivoire. Place and Duration of Study: This study was done in Institute Pasteur of Côte d’Ivoire between July 2019 and October 2019. Methodology: A total of 56 tubes used to store salmonella strains with few or no agar were analyzed to assess presence of Salmonella. The strains were first cultured in broth and then on selective agar (Hecktoen) medium and nutrient agar. Then, the Salmonella-specific InvA gene was directly detected in the tubes. Results: The results obtained showed that the quality of the Salmonella strains initially conserved for at least 10 years had clearly deteriorated because none of them had been cultured after enrichment and culture on agar media. However, 73.21% of tubes not containing storage agar have traces of Salmonella DNA followed by molecular identification. The preponderant contaminating bacterial flora was represented by Gram positive. Conclusion: These results should encourage all laboratories to proceed immediately with the quality control of their strain collections for excellent biobanking.
Aims: The overall objective of this study was to study antibiotic-resistant strains of Salmonella in livestock sales sites in the Abidjan district. Place and Duration of Study: Researchers team from Institute Pasteur of Ivory Coast and their students collected samples of cattle feces in five townships of the district of Abidjan from April to September 2016. Methodology: Fresh cow dung has been collected from sales outlets and livestock pens in five municipalities in Abidjan. The prevalence of Salmonella carrying has been studied by classical microbiological techniques. These included strain isolation by culture on the Hektoen medium, biochemical identification using Leminor's reduced rack and strain confirmation by MALDI-TOF MS. Phenotypic determination of antibiotic resistance and detection their genes were carried out respectively by the method of discs diffusion on the Muller-Hinton agar and using PCR simplex. Results: In this study, we collected 420 samples. The results showed that the overall prevalence of Salmonella isolated from cattle feceswas 20% (84/420). Twenty-six (26) strains were resistant to at least one antibiotic. The blaCTX-M, blaSHV, blaTEM and TetA resistance genes were detected with the respective frequencies of 7.7%, 57.7% and 7.7%. Conclusion: The isolation of antibiotic-resistant Salmonella strains in these healthy cattle poses a significant threat to public health. However, a good use of antibiotics in farms could help limit the phenomenon of resistance of Salmonella to antibiotics.
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