A improved, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated as per ICH guidelines for the quantification of acetic acid present in Gefitinib as an solvent impurity. Validation studies demonstrated that the proposed RP-HPLC method is simple, specific, rapid, reliable and reproducible. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the routine quality control analysis for the quantification of acetic acid in Gefitinib.
The aim of this study is to report the clinical features, imaging findings including confocal imaging, corneal nerve fiber analysis, and management outcomes in a series of three cases of varicella zoster virus (VZV) reactivation following one dose of coronavirus disease 2019 (COVID-19) vaccination. This was a retrospective and observational study. All the patients who developed uveitis post-vaccination were pooled together. Patients who had VZV reactivation were included. Two cases had polymerase chain reaction positive for VZV from aqueous humor. At the time of presentation, IgG and IgM spike protein antibodies for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) were tested. Out of this pool, three patients with classical features to describe pole-to-pole manifestations were chosen. A 36-year-old lady with post-vaccination sclerokeratouveitis associated with reactivation of herpes zoster ophthalmicus, a 56-year-old lady with post-vaccination acute anterior uveitis associated with herpes zoster ophthalmicus, and a 43-year-old gentleman with post-vaccination acute retinal necrosis were included. We present a possible link between anti-SARS-CoV-2 virus vaccination and varicella zoster reactivation in these patients and also describe the clinical features, imaging findings including confocal imaging, corneal nerve fiber analysis, and management with detailed discussion.
Carnation Mottle Virus (CarMV) is an important viral agent infecting carnation and causing considerable loss to this crop. The research was conducted to study the effect of extracts of medicinal plants on CarMV. The infected plants were treated with extracts of Asparagus sp., Ocimum spp. and Vitex sp. prepared in different solvents by soxhlet apparatus. Laboratory bio-assay as well as studies under field conditions was done to see their effect on viral diseases. The presence of virus was evaluated using DAS-ELISA and scoring was done through microtitre plate reader at 405 nm wavelength. Asparagus extract in acetone (AA) & methanol (AM) appears to exhibit maximum inhibitory potential against CarMV, when added to the medium after autoclaving at 10 mg/l concentration.There was no loss of inhibitory potential due to heat when this extract was added before autoclaving the medium. Asparagus extract in methanol (AM) was also effective at 10 mg/l. in both cases i.e. addition before and after autoclaving. Extract of Ocimum sanctum in water (OSW) was also found effective; however, its effectiveness was confined only to, after autoclaving, additions of the extract to the medium. Field spray on the other hand could not eliminate the virus under test. However, inhibition to certain degree was recorded.
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