As an emerging nano‐functional material, fluorescent carbon nanodots (CDs) have made breakthroughs in many fields. In our research, graphite‐like nitrogen‐doped carbon nanodots (N‐CDs) with excellent down‐ and up‐conversion fluorescent properties were synthesized by a facile one‐step solvothermal method using tri‐isopropanolamine (TIPA) as carbon and nitrogen sources. Mean particle diameter from 2.85 to 5.62 nm can be obtained by controlling the amount of HNO3 during synthesis. With low cytotoxicity and excellent fluorescence properties, the prepared N‐CDs can well label HeLa cells, which has great potential in the field of cell imaging. This work could enrich the research on the preparation and application of N‐CDs.
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