Allergic responses are mainly caused by IgE, which is often located on the cell surface. The current diagnostic method detects both allergen-specific IgE and total IgE levels, but a number of allergic patients have a normal serum IgE level, which is a poor clinical correlate for allergy. Here, we developed a simple method to detect the level of cell-bound IgE by dissociating it from blood cells with lactic acid. Dissociated cell-bound IgE and plasma IgE levels were detected using the same ELISA kit at the same time. We established two clinical cohorts: an allergic patient group and a healthy participant group. In general, cell-bound IgE correlated well with plasma IgE; however, some patients exhibited high cellbound IgE levels but low plasma IgE levels. We recommended 350 ng/mL peripheral blood total IgE (cell-bound ige + plasma IgE) as the cut-off value for allergy diagnosis. Using this indicator, 90.32% of our allergic patients were correctly diagnosed. The peripheral blood total IgE level is a promising clinical diagnostic indicator in allergic patients and will provide more guidance for allergy diagnosis and therapeutic evaluation.Immunoglobulin E (IgE) plays a key role in the development of allergic diseases 1,2 , and it is necessary to detect the IgE level for diagnosis and treatment evaluation 3 .The concentration of IgE in the circulation is very low (below 240 ng/ml in healthy individuals); it is the least prevalent antibody type, with a level much lower than the normal level of IgG (5-10 mg/ml) 4 . The half-life of free IgE in the blood is only 2-3 days, while IgE bound to the high-affinity receptor FcεRI on mast cells or basophils is stable for several weeks 5 . Most IgE is bound to cells through its receptors, leaving only a small proportion free in the plasma 6 . Serum IgE levels are very important for the diagnosis of allergies and generally correlate with disease severity 7 . However, the clinical detection of IgE is limited to free serum/plasma IgE, which ignores the large contribution of cell-bound IgE 8 . A number of allergic patients have normal serum IgE levels, which is why the World Allergy Association does not recommend the use of total IgE as a diagnostic guideline for allergy 9 . The level of free IgE in the blood is usually measured by ImmunoCAP 10 .Allergen-specific IgE is the causative agent of allergic disease. Several studies have reported that specific IgE levels correlate well with the severity of allergy; however, a relatively high number of molecules must be defined and produced at a sufficient quality to cover all clinically important allergen specificities 11 . Not all allergens that are in extracts have been defined at the molecular level yet. Other allergens have been well characterized but have not been produced at the quality level required for component-resolved diagnostic tests. The skin prick test is the gold standard for diagnostic allergy tests and is used to confirm allergic sensitization to suspected allergens and provide guidance for the treatment of patients. While...
This paper studies tuning heterogeneous transesterification catalysis and process for easy catalyst separation and enhanced reaction rate. Multibond metal alkoxides and ultrasonic pretreatment are employed to produce nanoemulsions with large interfacial area, which have the potential to be easily separated. With aluminum isopropoxide or titanium isopropoxide as the catalyst and surfactant, transparent alcohol/oil emulsions can be formed in less than four minutes and can significantly enhance the transesterification reaction rate. The micelle size was observed to be as low as 5.1 nm. Partially polymerized titanium isopropoxide also showed good catalytic activity and considerable amphiphilic properties in forming nanoemulsions. Viscosity and apparent vapor pressure reduction were also observed. The alcohol/soybean oil molar ratio was a main factor for apparent vapor pressure reduction.
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