There is no precedent for the application of rotary flow jetting tool in the drainage gas recovery under gas wells. Based on the motion principles of jet flow and vortex flow, this paper designs a downhole rotary flow jetting tool, and verifies the feasibility of the tool through fluid simulation. Next, an indoor experiment device was established for drainage gas recovery with rotary flow jetting tool by the principle of similarity, and the structural parameters of the tool were subject to multi-objective optimization through orthogonal experiments. The optimized tool can achieve ideal rotary flow height and discharge volume. The results show that the proposed rotary flow jetting tool can effectively separate gas from liquid, and produce a rotary flow. The optimal structural parameters were determined as follows: the pitch diameter of spiral body is 45mm, the throat pipe length is 247mm, the spiral angle is 55°-60°, the spiral wing width is 4-6mm, and the nozzle diameter is 15-25mm. In addition, the number of side holes of the throat pipe has little effect on the jetting effect.
Purpose: To investigate the potential role of miR-423-5p in osteoblastic differentiation of non-traumatic osteonecrosis of the femoral head (ONFH). Methods: MiR-423-5p levels in bone marrow samples from ONFH and osteoarthritis (OA) patients, respectively, were evaluated using quantitative (real-time) polymerase chain reaction (qPCR). Osteoblastic differentiation was monitored using Alizarin red S staining, while cell viability was determined by MTT assay in hMSC-BM. MiR-423-5p expression was also measured during osteoblastic differentiation. The underlying mechanisms were explored using TargetScan database, and a series of in vitro experiments was performed to confirm this. Results: MiR-423-5p levels were significantly upregulated in ONFH samples (p < 0.01) and miR-423-5p expression significantly increased in human mesenchymal stem cells-bone marrow (hMSC-BM) after bone morphogenetic protein 2 (BMP-2) treatment. Furthermore, miR-423-5p downregulated osteoblastic differentiation and suppressed cell viability. Furthermore, SMAD3 was observed to be a downstream target of miR-423-5p via bioinformatics analysis; further in vitro experiments confirmed this. Conclusion: MiR-423-5p downregulates osteoblastic differentiation and cell viability by targeting SMAD3 in non-traumatic osteonecrosis. Thus, MiR-423-5p may serve as a potential target for promoting osteoblastic differentiation in ONFH patients.
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