The full-length cDNA sequence of F3′5′H gene from the Brunfelsia acuminata was obtained by RT-PCR and RACE, whose GenBank accession number is JQ678765. The sequence contains a 1521 bp open reading frame, 120 bp 5′UTR and 61 bp 3′UTR, encoding a total of 506 amino acids. The molecular mass of the predicted protein is 56.47 kDa with an estimated pI of 8.78, respectively. Sequence alignment showed that the amino acid sequence of F3′5′H was 91%, 87% and 84% with that of Petunia × hybrida, Nierembergia sp., Solanum tuberosum, respectively. Real-time quantitative PCR analysis showed that the expression of F3′5′H gene was different in petals of different days, which was the highest expression level on day 0 and significantly higher than other days. The results indicated that F3′5′H might play key role in flower color regulation and provide a theoretical reference for blue flower molecular breeding.
As an emerging technology, shoot encapsulation has been employed in germplasm conservation, distribution, and micropropagation of elite plant species. However, the production of synthetic seeds of sweet potato via non-zygotic embryogenesis requires a large number of embryos per cultured callus suspension and is labour-intensive. Here, we reported a simple method of encapsulating in vitro derived vegetable sweet potato nodal segments with sodium alginate, calcium chloride (CaCl2), and Murashige and Skoog (MS) salts. The nodes encapsulated with 4% sodium alginate (w/v) and 100 mM CaCl2 were the most suitable for propagation. They had uniform spherical beads and took the least number of days to shoot and root emergence. These plantlets produced more leaves, roots, and long shoots. Further evaluation of the MS salts concentration revealed that the plantlets encapsulated and grown with ½ MS salts had the least days to shoot and root emergence. They also had a longer shoot, the highest conversion rate (99%), and the least leaf abscission (17%). Thus, the sweet potato nodal segments encapsulated with 4% sodium alginate, 100 mM CaCl2, and ½ MS salts could be used as excellent material for micropropagation, germplasm conservation, and exchange of sweet potato planting materials.
Three cultivars of pomelo “Guanximiyou” (GX) and its variants “Hongroumiyou” (HR) and “Sanhongmiyou” (SH) were selected as experimental materials to evaluate the quality and edible value of the fruit. In this study, the fruit quality and lycopene contents were investigated. The results show that among the 3 cultivars used, the contents of soluble solids, vitamin C, and total phenol in the juice sac of SH were the highest, followed by HR and GX. SH has the largest fruit shape index, soluble sugar content: GX > HR > SH, and titratable acid content: GX < HR < SH. From our findings, the content of lycopene was the highest in SH, followed by HR and GX. SH has the greatest potential for production due to its appreciable content of lycopene and fruit quality.
The fruits of myrtle at different stages of development, namely green (G), colour break (C) and mature (M), were used to investigate the changes of fruit quality and anthocyanin composition during its development. Five anthocyanin components, such as delphinidin (Dp), cyanidin (Cy), pelargonidin (Pg), peonidin (Pn) and malvidin (Mv), were detected. Among them, the content of Dp glucoside was the highest (2.12 μg · g−1) and the content of Pn glucoside was the lowest (0.17 μg · g−1) at the green stage. The content of Cy glucoside was the highest (120.94 and 1,200.03 μg · g−1), and the content of Mv glucoside was the lowest (19.50 and 62.72 μg · g−1) at the colour break and mature stages. The single fruit weight, vertical diameter and transverse diameter at the mature stage were significantly higher than those at the colour break and the green stages by 0.87 g, 1.27 mm, 1.53 mm and 1.54 g, 3.4 mm, 3.55 mm, respectively. Fruit quality, the contents of soluble sugar and carotenoids, gradually increased with the development of fruit; and the contents were the highest at the mature stage (17.68% and 16.90 μg · L−1). The contents of titratable acidity, total phenol, flavonoids, chlorophyll a and total chlorophyll gradually decreased with the development of fruit, the content was the lowest at the mature stage (2.71 mmol · 100 g−1, 9.29 μg · g−1, 0.1%, 0.37 μg · L−1, 0.9 μg · L−1) and the content was the highest at the green stage (5.25 mmol · 100 g−1, 21.07 μg · g−1, 0.17%, 8.86 μg · L−1, 15.47 μg · L−1). The difference between the green period and the colour break period was significant.
In this study, the changes in quality parameters and sensory-influencing parameters from the peel, red flesh, and white flesh of ‘Yonglian No.1′ peach fruits were analyzed during cold storage. The results indicated that the contents of total soluble solids (TSS), soluble sugar, organic acid, vitamin C, total anthocyanin, phenol, and flavonoids, as well as the good fruit rate varied depending on the storage stages and storage treatments. The peach fruits in MAP stored for 50 days had favorable exterior qualities, a good fruit rate of 100%, and a higher content of total soluble solids (TSS) at 12.6%. MAP was significantly effective at maintaining fruit firmness, the content of TSS, soluble sugar, organic acid, vitamin C, total anthocyanin, phenol, and flavonoids. Among the derivatives of anthocyanin, both cyanidin and pelargonidin were found in the peel, with a content of 33.45 mg/kg FW and 1.82 mg/kg FW, respectively. However, cyanidin was detected in the flesh with a content of 40.42 mg/kg FW. In the present work, the differences regarding phytochemical profiles and physical properties were mainly correlated with the storage stages and storage treatments of peach fruit. ‘Yonglian No.1′ had higher levels of health-promoting compounds during storage and maintained favorable quality.
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