Ag nanoparticles were successfully prepared using a liquid reduction method with a suitable mixture reductant of polyethylene glycol (PEG) and ethylene glycol (EG). OP-10 as a dispersing agent, was used to prepare the conductive Ag ink. Ag nanoparticles with an average particle size of 40 nm were prepared while the ratio of PEG to EG was 1:2. Meanwhile, the Ag particles had a narrow size distribution and great dispersion performance. The effects of paper substrates, sintering temperature, and sintering time on the conductivity of the printed Ag ink pattern were also studied. It was found that Lucky porous high glossy photo paper was a good candidate as the printing substrate. The resistivity of the printed pattern could reach 5.1 × 10−3 Ω·cm after heated at 100 °C for 2 h. Hence, the printed pattern showed good conductivity which led to the LED light being on. Furthermore, the Ag nanoparticle ink could be printed to form any pattern as required that still showed good electrical conductivity after being sintered under low-temperature. This could provide new possibilities for the preparation of flexible electrodes.
The present study describes the preparation of a dodecapeptide YHWYGYTPQNVI (GE11)-conjugated liposome bound with polyethylene glycol to enhance the therapeutic effect of resveratrol (RSV) in head and neck cancer cells. The results indicated that (RSV)-loaded GE11-conjugated liposomes (RSV-GL) exhibited a high entrapment efficiency of >95%, with an active drug loading level of 19.5% w/w. Release kinetics revealed that RSV was released in a slow and sustained manner from the RSV-GL and RSV-loaded liposome (RSV-L) nanoparticulate systems. The epidermal growth factor receptor (EGFR)-overexpressing squamous cell carcinoma HN cells specifically internalized GE11 surface-conjugated liposome in a manner that was markedly increased compared with that of the non-targeted carrier. Consistently, RSV-GL exhibited a significantly increased cytotoxic effect compared with that of the non-targeted nanoparticles. Notably, RSV-GL induced significantly increased proportions of early (~60%) and late (~10%) apoptotic cells in head and neck cancer cell populations. To the best of our knowledge, the application and development of EGFR-targeted peptide-conjugated liposome system for RSV delivery has not been studied previously in the treatment of head and neck cancer. In addition, RSV-GL exhibited the greatest antitumor efficacy compared with any other group. RSV-GL exhibited a 2-fold decrease in tumor volume compared with the free RSV and a 3-fold decrease in volume compared with the control. Overall, the nanomedicine strategy described in the present study may potentially advance the chemotherapy-based treatment of head and neck cancer, with promising applications in other EGFR-overexpressing tumors.
Abstract. In this work, using chemical reduction method, we synthesized silver particles with different sizes and morphologies by simply changing the proportion of the associative reducing agents. More specifically, when the reducing agent is ascorbic acid, the morphologies of silver particles are quasi-spherical shapes, the diameter size of silver nanoparticles was in the range from 200 nm to 300 nm. When the reducing agent is hydrogen peroxide, the morphologies of silver particles are irregular bulks, the diameter size of silver nanoparticles was in the range from 1 um to 2 um. When the volume ratio of ascorbic acid (0.09M) and hydrogen peroxide (0.98M) is 1: 1, the morphologies of silver particles are unique, they are coral clusters reunited by long silver nanoplates.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.