A new method of protein detection was explored on the resonant Raman scattering signal of ZnO nanoparticles. A probe for the target protein was constructed by binding the ZnO/Au nanoparticles to secondary protein by eletrostatic interaction. The detection of proteins was achieved by an antibody-based sandwich assay. A first antibody, which could be specifically recognized by target protein, was attached to a solid silicon surface. The ZnO/Au protein probe could specifically recognize and bind to the complex of the target protein and first antibody. This method on the resonant Raman scattering signal of ZnO nanoparticles showed good selectivity and sensitivity for the target protein.
In this paper, near-infrared (NIR) fluorescent CuInS 2 QDs@SiO 2 nanobeads were prepared and used as fluorescent nanoprobes for prostate cancer cells imaging. The core-shell CuInS 2 QDs@SiO 2 nanobeads with controllable particle sizes were synthesized via a reverse microemulsion method. Further surface modifications were performed for grafting amino groups on the surface of the NIR CuInS 2 QDs@SiO 2 nanobeads. For prostate cancer cell imaging, anti-PSCA antibody was conjugated to the NIR CuInS 2 QDs@SiO 2 nanobeads to prepare the anti-PSCA-QDs@SiO 2 nanoprobe. The specific binding of the antibody conjugated CuInS 2 QDs@SiO 2 nanobeads to the surface of human prostate cancer cells (PC-3M) was confirmed by fluorescence microscopy. MTT assay and fluorescence microscopy images showed that the anti-PSCA-conjugated NIR CuInS 2 QDs@SiO 2 nanoprobe was a non-toxic nanoprobe and had high-specificity in cell imaging. The CuInS 2 QDs@SiO 2 nanoprobe as an efficient NIR imaging nanoprobe could be used for target imaging, biological assays and early diagnosis of cancer.
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