mediates an essential event for antigen-specific T cell activation and an important mechanism for mounting an immune response.6) Thus, CCR7 could be a potential target for treatment of various metastases and/or inflammatory conditions.In this study, a fraction from a sponge was identified which showed inhibitory activity in the CCR7 receptor In the primary HTS assay, a sample in the MFL from a active fraction was subjected to further purification. The preliminary CG161 fractions were prepared as previously described.4,8) In this study, fraction 3 (50% acetonitrile (ACN) elution) was active in the CCR7 binding assay. Fraction 3 (35mg) was further purified on an HPLC ACN gradient system (15ml/minute, 2% to 30% ACN over 40 minutes, and 30% to 70% ACN over next 40 minutes), to yield -100 fractions (13ml/fraction). Two pure compounds 2 (1.8mg) and 1 (0.9mg) were obtained with retention time -54 minutes and -57 minutes, respectively. Detailed NMR analysis9) idetified 2 as sulfircin, a compound whose structure had been determined previously by X-ray crystallography.10)The structure of 1 was determined based on extensive NMR and HRMS analyses and by comparison to 2.11) From the high-resolution negative ion ESI-MS, the molecular formula of 1 was established as C25H39O5S (found m/z 451.2529; calcd. 451.2523 for [M]-). All spectral data including the HRMS, 1H and 13C NMR data strongly suggested that 1 was structurally related to 2. The 13C
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