Rapid and early diagnosis
of respiratory viruses is key to preventing
infections from spreading and guiding treatments. Here, we developed
a sensitive and quantitative surface-enhanced Raman scattering-based
lateral flow immunoassay (SERS-based LFIA) strip for simultaneous
detection of influenza A H1N1 virus and human adenovirus (HAdV) by
using Fe3O4@Ag nanoparticles as magnetic SERS
nanotags. The new type of Fe3O4@Ag magnetic
tags, which were conjugated with dual-layer Raman dye molecules and
target virus-capture antibodies, performs the following functions:
specific recognition and magnetic enrichment of target viruses in
the solution and SERS detection of the viruses on the strip. Based
on this strategy, the magnetic SERS strip can directly be used for
real biological samples without any sample pretreatment steps. The
limits of detection for H1N1 and HAdV were 50 and 10 pfu/mL, respectively,
which were 2000 times more sensitive than those from the standard
colloidal gold strip method. Moreover, the proposed strip is easy
to operate, rapid, stable, and can achieve high throughput and is
thus a potential tool for early detection of virus infection.
The world‐wide spreading of coronavirus disease (COVID‐19) has greatly shaken human society, thus effective and fast‐speed methods of non‐daily‐life‐disturbance sterilization have become extremely significant. In this work, by fully benefitting from high‐quality AlN template (with threading dislocation density as low as ≈6×10
8
cm
−2
) as well as outstanding deep ultraviolet (UVC‐less than 280 nm) light‐emitting diodes (LEDs) structure design and epitaxy optimization, high power UVC LEDs and ultra‐high‐power sterilization irradiation source are achieved. Moreover, for the first time, a result in which a fast and complete elimination of SARS‐CoV‐2 (the virus causes COVID‐19) within only 1 s is achieved by the nearly whole industry‐chain‐covered product. These results advance the promising potential in UVC‐LED disinfection particularly in the shadow of COVID‐19.
Facile and simple method is developed to synthesize silver-nanoparticle-decorated quercetin nanoparticles (QA NPs). Modification suggests that synergistic quercetin (Qe) improves the antibacterial effect of silver nanoparticles (Ag NPs). Characterization experiment indicates that QA NPs have a diameter of approximately 10 nm. QA NPs show highly effective antibacterial activities against drug-resistant Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). We explore antibacterial mechanisms using S. aureus and E. coli treated with QA NPs. Through morphological changes in E. coli and S. aureus, mechanisms are examined for bacterial damage caused by particulate matter from local dissociation of silver ion and Qe from QA NPs trapped inside membranes. Moreover, we note that gene expression profiling methods, such as RNA sequencing, can be used to predict discover mechanisms of toxicity of QA NPs. Gene ontology (GO) assay analyses demonstrate the molecular mechanism of the antibacterial effect of QA NPs. Regarding cellular component ontology, "cell wall organization or biogenesis" (GO: 0071554) and "cell wall macromolecule metabolic process" (GO: 0044036) are the most represented categories. The present study reports that transcriptome analysis of the mechanism offers novel insights into the molecular mechanism of antibacterial assays.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.