Background: It is reported that miR-596 has a potential diagnostic value for non-traumatic osteonecrosis of the femoral head (NOFH), but its underlying mechanisms in NOFH is unclear. Methods: The expression of miR-596 and Smad3 was detected by western blot and quantitative real-time PCR. The relationship between the two molecules was explored using Dual-Luciferase Reporter Assay. Glucocorticoid (GC)dexamethasone, was used to induce bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation, and the effects of miR-596 on BMSC osteogenic differentiation and proliferation were determined.Results: MiR-596 expression was upregulated, while Smad3 expression was inhibited in the bone marrow samples of patients with steroid-induced osteonecrosis of femoral head (SANFH). Overexpression of miR-596 inhibited the proliferation and osteogenic differentiation of BMSCs induced by GC. Meanwhile, the opposite results were observed in the miR-596 inhibitor group. In addition, Smad3 was a target gene of miR-596, and negatively regulated by miR-596. The promotion effect of the miR-596 inhibitor on BMSC proliferation and osteogenic differentiation was reversed by si-Smad3.Conclusion: MiR-596 can suppress GC-BMSC osteoblastic differentiation and proliferation by regulating Smad3 expression.
With the wide application of Internet technology, enterprises have formed a large amount of data in the production and operation process. By using big data technology to realize the sharing of supply chain information between enterprises, it plays an important role in improving the operating efficiency of enterprises. This paper first analyzes the concept of regular supply chain information sharing, shared subjects, shared content, and typical sharing patterns. On this basis, research on the application of big data to supply chain information sharing, research on content sharing, information acquisition, different data conversion processing and application methods of supply chain information sharing based on big data, especially based on large The data sharing chain information sharing model is constructed and analyzed, and the theoretical system and application mode of enriching the information sharing of the entire supply chain are discussed.
In recent years, the rapid development of modern information technology has promoted the reform of education and teaching methods. The integration of modern information technology and traditional teaching methods has formed a new blend-teaching model. At present, the new blend-teaching mode has been widely used, and some problems have arisen. Through the practice, analysis and comparison methods, this paper analyzes how to improve the scientific, rationality, pertinence, effectiveness and comprehensive evaluation of the blend-teaching mode. On this basis, it puts forward 11 articles that need further improvement. It is hoped that through the research on the problems existing in the application of the new blend-teaching mode, some teachers will be provided to help teachers who use the blend-teaching mode.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.