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Coupled-dipole approximation (CDA) calculations of microwave extinction and radar backscatter are presented for nonhomogeneous (soft) ice spheres and for quasi-realistic aggregates of elementary ice crystal forms, including both simple needles and real dendrites. Frequencies considered include selections from the Dual-Frequency Precipitation Radar (DPR; 13.4 and 35.6 GHz) and the Global Precipitation Measurement (GPM) Microwave Imager (GMI; 18.7, 36.5, and 89.0 GHz), both slated for orbit on the GPM mission.The computational method is first validated against Mie theory using dipole structures representing solid ice spheres as well as stochastically generated ''soft'' ice spheres of variable ice-air ratio. Neither the traditional Bruggeman nor Maxwell Garnett dielectric mixing formula is found to correctly predict the full range of CDA results for soft spheres. However, an excellent fit is found using the generalized mixing rule of Sihvola with n 5 0.85.The suitability of the soft-sphere approximation for realistic aggregates is investigated, taking into account the spectral dependence of backscatter and/or extinction per unit mass at key DPR and GMI frequencies.Even when spheres of nonequal mass are considered, there is no single combination of fraction and mass that simultaneously captures all the relevant radiative properties. All four aggregate models do, however, exhibit a predictable power-law dependence of the mass extinction coefficient on the total particle mass. Dual-frequency mass extinction ratios are only very weakly dependent on particle masses; moreover, the ratio is found to be approximately proportional to frequency raised to the power 2.5.The dual-frequency backscatter ratio is found to be a predictable function of the aggregate mass for particles smaller than 3 mg. Above this size, the ratio is strongly sensitive to aggregate shape, a finding that raises concerns about the utility of dual-frequency backscatter ratio measurements whenever larger particles might be present in a volume of air.The validity of the Rayleigh-Gans approximation applied to radar backscatter from snow aggregates was also examined. Although the dual-frequency backscatter ratio was reasonably well reproduced, the absolute magnitude was not.

The four-parameter modified gamma distribution (MGD) is the most general mathematically convenient model for size distributions of particle types ranging from aerosols and cloud droplets or ice particles to liquid and frozen precipitation. The common three-parameter gamma distribution, the exponential distribution (e.g., Marshall–Palmer), and power-law distribution (e.g., Junge) are all special cases. Depending on the context, the particle “size” used in a given formulation may be the actual geometric diameter, the volume- or area-equivalent spherical diameter, the actual or equivalent radius, the projected or surface area, or the mass. For microphysical and radiative transfer calculations, it is often necessary to convert from one size representation to another, especially when comparing or utilizing distribution parameters obtained from a variety of sources. Furthermore, when the mass scales with Db, with b < 3, as is typical for snow and ice and other particles having a quasi-fractal structure, an exponential or gamma distribution expressed in terms of one size parameter becomes an MGD when expressed in terms of another. The MGD model is therefore more fundamentally relevant to size distributions of nonspherical particles than is often appreciated. The central purpose of this paper is to serve as a concise single-source reference for the mathematical properties of, and conversions between, atmospheric particle size distributions that can expressed as MGDs, including exponential and gamma distributions as special cases. For illustrative purposes, snow particle size distributions published by Sekhon and Srivastava, Braham, and Field et al. are converted to a common representation and directly compared for identical snow water content, allowing large differences in their properties to be discerned and quantified in a way that is not as easily achieved without such conversion.

Complement synthesis in cells of origin is strongly linked to the pathogenesis and progression of renal disease. Multiple studies have examined local C3 synthesis in renal disease and elucidated the contribution of local cellular sources, but the contribution of infiltrating inflammatory cells remains unclear. We investigate the relationships among C3, macrophages and Th17 cells, which are involved in interstitial fibrosis. Here, we report that increased local C3 expression, mainly by monocyte/macrophages, was detected in renal biopsy specimens and was correlated with the severity of renal fibrosis (RF) and indexes of renal function. In mouse models of UUO (unilateral ureteral obstruction), we found that local C3 was constitutively expressed throughout the kidney in the interstitium, from which it was released by F4/80+macrophages. After the depletion of macrophages using clodronate, mice lacking macrophages exhibited reductions in C3 expression and renal tubulointerstitial fibrosis. Blocking C3 expression with a C3 and C3aR inhibitor provided similar protection against renal tubulointerstitial fibrosis. These protective effects were associated with reduced pro-inflammatory cytokines, renal recruitment of inflammatory cells, and the Th17 response. in vitro, recombinant C3a significantly enhanced T cell proliferation and IL-17A expression, which was mediated through phosphorylation of ERK, STAT3, and STAT5 and activation of NF-kB in T cells. More importantly, blockade of C3a by a C3aR inhibitor drastically suppressed IL-17A expression in C3a-stimulated T cells. We propose that local C3 secretion by macrophages leads to IL-17A-mediated inflammatory cell infiltration into the kidney, which further drives fibrogenic responses. Our findings suggest that inhibition of the C3a/C3aR pathway is a novel therapeutic approach for obstructive nephropathy.

Endogenous ANXA1 expression at the DRG level is involved in CFA-induced inflammatory pain, and i.t. ANXA1 20 µg µl(-1) produces its anti-nociceptive effect through FPR2/ALX.

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N-acetylcysteine has been widely used as a nutritional supplement and drug in humans for its antioxidant properties. The complement activation fragment C5a is a strong proinflammatory molecule that mediates cell adhesion, chemotaxis, and the complex biological functions. However, the effect of NAC on the C5a, and the relationship of those two with cisplatin-induced acute kidney injury are unknown. In cisplatin induced AKI mouse model, mice with NAC administration had a marked improvement in renal function (BUN and Cr), decreased pathological damage, reduced inflammation, and alleviated renal oxidative stress. Furthermore, C5a and C5aR expression in the cisplatin-treated group was notably increased compared with the control group, and this increase could be significantly inhibited by NAC. In addition, neutrophils coexpressed distinctly with C5aR, and the number of infiltrating neutrophils (MPO+ly6G+) and inflammatory factors decreased with NAC treatment in the cisplatin-treated group. Overall, these data demonstrate that NAC could ameliorate cisplatin-induced nephrotoxicity in mice and the protective effects may be conducted by inhibiting the activation of kidney inflammation and the complement system.

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