The aim of this study was to evaluate Egyptian date palm pollen (DPP) grains composition, physical and functional potentials in comparing with two forms; 80% ethanol extract, and nanoencapsulated form. Functional yoghurt fortified with DPP in three forms was prepared and their physicochemical, microstructure, texture and sensory characteristics were assessed. The micro morphology was explored via Scanning Electron Microscope (SEM). Fourier Transform Infrared (FTIR) spectroscopy was employed for functional groups detection. Phenolic compounds were detected by High Performance Liquid Chromatography (HPLC) while fatty acids were identified via Gas Liquid Chromatography (GLC). Cytotoxicity of DPP nanocapsules was evaluated against RPE1 cell line (BJ1). The Egyptian date palm pollen grains evaluation revealed its rich content of protein and carbohydrate (36.28 and 17.14 g/ 100g), high content of Fe, Zn and Mg (226.5, 124.4 and 318 mg/100g), unsaturated fatty acids ω-3, ω-6 and ω-9 (8.76, 20.26 and 7.11 g/100g, which was increased by ethanol extraction) and phenolic compounds especially catechin (191.73 μg/mL) which was pronounced in DPP antioxidant potentials (IC50 35.54 mg/g). The FTIR analyses indicated the presence of soluble amides (proteins) and polysaccharides (fibers) functional groups in DPP. Fortification with nanoencapsulated DPP proved to be safe and the recommended form due to the announced positive characteristics. Yoghurt fortification with DPP forms enhanced viscosity, syneresis and Water Holding Capacity (WHC), which can be considered a symbiotic functional product as it contained both probiotics (106 CFU/g) and prebiotics represented in DPP forms.

Iron deficiency anemia (IDA) is a major health concern in developing countries, and these see an increased incidence in pregnant women and children in particular. The contribution of dairy products as natural products in drug delivery approaches is inspiring. This study aimed to analyze the application of iron (Fe) and folic acid (FA) bovine serum albumin-nanoparticles (BSA-NPs) as anti-anemic pharmacological agents that fortify stirred functional yogurt (SFY), comparing these with a plain control and SFY fortified with Fe and FA in free forms. The physicochemical, cytotoxicity, microbiological, viscosity, oxidative interactions, microstructural, sensorial analyses, and bioavailability properties of IDA-induced Albino rats were examined. The Transmission Electron Microscope (TEM), Zetasizer, and Scan Electron Microscope (SEM) were applied. Nanocapsule-fortified SFY showed an enhanced apparent viscosity, water-holding capacity, microstructure, least lipid oxidation, and overall sensorial acceptability. Feed that included Fe + FA nanocapsule-fortified SFY (G6) succeeded in restoring hemoglobin (16.53 gdL−1), iron (109.25 μgdL−1), ferritin (33.25 μgdL−1), and total protein (8.6 gdL−1) at the end of the 4-week feeding period, with significant competition revealed in calcium and zinc absorbance. Nanocapsule-fortified SFY showed no adverse effects or architectural alterations in the liver, kidney, or spleen, as indicated by biochemical and histological examinations. Bovine serum albumin-nanoparticles (BSA-NPs) of iron (Fe) and folic acid (FA) can be recommended as anti-anemia supplements in different functional food applications.

The objective of the present study was to utilize whey and permeate as the by-products of the cheese industry after mixing with kumquat purée and/or paste for producing functional beverages. Physical, chemical, physicochemical, microbiological and sensory properties of kumquat fruit and its purée and paste were studied. The results indicated that kumquat purée and paste are good sources of β-carotene, vitamin C, minerals such as calcium, potassium and magnesium, total phenolic acids and flavonoids. In addition, they have high antioxidant capacity. Physicochemical, microbiological and sensory properties of the prepared functional beverages during cold storage periods were determined. The obtained results indicated that ascorbic acid and β-carotene of all beverages were higher than those of whey and permeate only, with a high level of total phenols, flavonoid content and antioxidant activity. Generally, sensory evaluation of the prepared beverages showed that the addition of purée and paste of kumquat fruit to whey and permeate increased the overall acceptability of these beverages. Total count was in permissible limit while the coliform and moulds & yeasts counts were not detected in all beverages during storage at 4±1°C for 28 days. Beverages containing purée and paste of kumquat fruit could be recommended as new acceptable functional products.

Synbiotic products contain both probiotic bacteria and prebiotic compounds. The aim of this study was to produce a symbiotic Fermented dairy product with the polysaccharides / fibers (maltodextrin, polydextrose and inulin) with a 3% concentration in combining them with Bifidobacterium longum ATCC 15707, Streptococcus thermophiles Texl 5842 and Lactococcus lactis subsp lactis ATCC 53214 (Lac. lactis spp lactis) in either skimmed or full fat fermented milk, and testing, the sensory and physicochemical and microbiological properties for fresh product and after incubation for 7 days. It was shown that the pH was having a clear decrease and acidity was showing an increase that was lowered by increasing the storage days at 5-7°C as well as a significant decreasing in the syneresis and increase in log 10 cfu/g of B. longumATCC 15707 count and viscosity was markedly increased at maltodextrin treatment. Finally, it was concluded that Maltodextrin was found to have good results in stimulation the probiotic bacterial count and enhancing the sensory properties of fermented milk compared with inulin and that is recommended by our work to use it in industry.

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