Magnetically responsive heparin-immobilized cellulose nanofiber composites were synthesized by wet-wet electrospinning from a nonvolatile, room-temperature ionic liquid (RTIL), 1-methyl-3-methylimidazolium acetate ([EMIM]-[Ac]), into an aqueous coagulation bath. Superparamagnetic magnetite (Fe 3 O 4 ) nanoparticles were incorporated into the fibers to enable the manipulation of both dry and wet nanofiber membranes with an external magnetic field. Three synthetic routes were developed to prepare three distinct types of nanocomposite fibers: cellulose-Fe 3 O 4 −heparin monofilament fibers, cellulose-Fe 3 O 4 −heparin core−shell fibers with heparin covalently immobilized on the fiber surface, and cellulose -Fe 3 O 4 core−shell fibers with heparin physically immobilized on the fiber surface. These nanocomposite fibers were characterized by electron microscopy to confirm their coaxial structure and the fiber dimensions (fiber diameter 0.2−2.0 μm with 0.1−1.1 μm core diameter). Thermogravimetric analysis, liquid chromatography−mass spectrometry, Fourier transform infrared and X-ray diffraction spectroscopy provided detailed compositional analysis for these nanocomposite fibers, confirming the presence of each component and the surface accessibility of the heparin. The anticoagulant activity of immobilized heparin on the nanocomposite fiber surfaces was evaluated and confirmed by antifactor Xa and antifactor IIa assays.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.