Centipeda minima has been used since centuries as a traditional medicinal plant in treating a number of disease conditions, the bioactive components responsible for its anti-inflammatory and anti-arthritis activity have not been identified. The present study was planned to isolate flavonoids fractions from Centipeda minima leaves extracts, and assessed anti-inflammatory and anti-arthritis activity in rats. The hydroalcoholic and aqueous extracts of Centipeda minima leaves were prepared and evaluated for in vitro antioxidant activity namely DPPH, total polyphenol content, total flavonol content and reducing power assay. The different fractions were isolated from hydroalcoholic extracts by using column chromatography. The Flavonoids fractions were investigated for anti-inflammatory and anti-arthritis activity in carrageenan rat induced paw oedema, cotton pellet-induced granuloma model and adjuvant induced chronic arthritis in rats. The findings of in vitro antioxidant activity confirmed that hydroalcoholic extracts expressed higher antioxidant activity compared to aqueous extract, and hence hydroalcoholic extracts was further selected for the isolation of various fractions. The results of phytochemical study suggest that FCM6, FCM7 and FCM8 exposed the presence of polyphenol and flavonoids. The fraction FCM6, FCM7 and FCM8 (25 mg/kg) exhibits significant antiinflammatory and anti-arthritis activity. The outcomes suggest that the anti-inflammatory and anti-arthritis activity of isolated fraction was due to presence of flavonoids and polyphenols.
Background: Detection of left ventricular (LV) dysfunction in anemic patients using 2D strain echocardiography has been investigated inadequately.
Aims and Objectives: This study was designed to compare the echocardiographic characteristics in patients with severe anemia to those without anemia using a 2D strain pattern.
Materials and Methods: A cross-sectional study was conducted between April 2021 and March 2022. A total of 100 patients were incorporated in the case group, where patients with hemoglobin levels <7 g/dL, aged between 18 and 60 years, were included, whereas 100 patients with normal hemoglobin levels were included in the control group. Baseline clinical characteristics and echocardiographic parameters were studied.
Results: The mean age of the case group was 38.32±14.9 years and of the control group was 39.94±14.3 years. In each group, majority of the patients were females (55%) and were from rural area. Mean hemoglobin level was 4.81±1.42 g/dL in case group and 12.41±0.9 g/dL in control group (P=0.0024). Mean peak E velocity (P=0.0002), mean peak A velocity (P<0.0001) and mean isovolumetric relaxation time (P=0.4612) represented statistically significant differences among case and control groups. The ejection fraction was 58% in the case and 56% in control group (P=0.621). Stroke volume was 58 mL in the case and 52 mL in the control group (P=0.653). LV dysfunction represented a significant difference between case and control group (Chi=28.125; P=0.0001). Furthermore, a statistically significant difference was observed between the global longitudinal strain score of the case and the control group (P=0.0015).
Conclusion: Using 2D strain imaging technique, LV dysfunction was detected in severely anemic patients who had no previously detected severe cardiovascular diseases.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.