Premarital screening in extended family members, followed by prenatal diagnosis is acceptable and the most effective strategy for control of thalassemia in developing countries like India.

Flow-cytometric osmotic fragility test is the best possible single test followed by EMA for diagnosis of HS. A combination of FOFT and EMA can correctly diagnose 100% patients. These tests are likely to replace conventional OFTs in future.

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Objectives: The term TMD refers to a group of disorders characterized by pain in the temporomandibular joint and associated structures. The aim of this study was designed to evaluate prevalence, severity and sex distribution of sign and symptoms of TMD and to evaluate their relation with anxiety and depression among the students. Material and Methods: A total of 1000 university students were enrolled in the study (550 females; 450 males), with ages ranged between 18 and 28 years. Helkimo anamnestic index (Ai) and clinical dysfunction index (Di) were used to determine symptoms and signs respectively. For the association of TMD with anxiety and depression, HAD (Hospital Anxiety and Depression) scale was used. Results showed that prevalence of one or more symptoms of TMD was 27.7%, while the prevalence of one or more signs of TMD was 64.4% which was mild in severity. Mild anamnestic symptoms (AiI) were found in 19.8% and severe symptoms (AiII) were found in 7.6%, while mild clinical sign (DiI), moderate clinical (DiII) and severe clinical sign (DiIII) were found in 49.7%, 12.2% and 2.4% respectively. Statistically there was no gender difference in these two scales. Regarding the association between TMD with anxiety and depression, 206 of the 311 students (66.2%) with TMD symptoms also had signs of anxiety and depression (P < 0.001). Conclusion: These findings confirmed that students had high prevalence of TMD which was significantly associated with anxiety and depression.

Microcytic hypochromic anemia is a common condition in clinical practice and alpha-thalassemia has to be considered as a differential diagnosis. Molecular diagnosis of alpha-thalassemia is possible by polymerase chain reaction. The aim of this study was to evaluate the frequency of alpha-gene numbers in subjects with microcytosis. In total, 276 subjects with microcytic hypochromic anemia [MCV<80fl; MCH<27pg] were studied. These include 125 with thalassemia trait, 48 with thalassemia major, 26 with sickle-cell thalassemia, 15 with E beta-thalassemia, 40 with iron-deficiency anemia, 8 with another hemolytic anemia, and 14 patients with no definite diagnosis. Genotyping for -alpha3.7 deletion, -alpha4.2 deletion, Hb Constant Spring, and a-triplications was done with polymerase chain reaction. The overall frequency of -alpha3.7 deletion in 276 individuals is 12.7%. The calculated allele frequency for a-thalassemia is 0.09. The subgroup analysis showed that co-inheritance of a-deletion is more frequent with the sickle-cell mutation than in other groups. We were able to diagnose 1/3 of unexplained cases of microcytosis as a-thalassemia carriers. The a-gene mutation is quite common in the Indian subcontinent. Molecular genotyping of a-thalassemia helps to diagnose unexplained microcytosis, and thus prevents unnecessary iron supplementation.