Objective: The objective of this study was to isolate and identify the fungal agents obtained from clinical samples of suspected mycoses patients attending Yenepoya hospital.Methods: Various clinical samples obtained from patients were subjected to preliminary examination according to standard mycological protocols such as KOH mount, wet mount, Gram’s staining, and Indian ink examination. Causative organisms were identified by macroscopically and microscopically after growth on sabouraud’s dextrose agar (with or without cycloheximide and chloramphenicol) and confirmed by lactophenol cotton blue mount, slide culture technique, urease test, and growth on chrome agar accordingly.Results: Of 274 clinical samples, 125 were culture positive in which the fungal isolates obtained were dermatophytes (all 3 genera), Candida species (including Candida albicans), Fusarium species, Aspergillus fumigatus, and Cryptococcus neoformans. Male predominance was found among the patients being 6:3 male:female ratio.Conclusion: Among the superficial infection, Trichophyton mentagrophytes was found to be predominant isolates; however, in systemic infection, C. albicans was the predominant isolates.
In order to determine the extent and causative agent of ringworm infections in Jaipur area, a study was done in outdoor patient Department of Skin, SMS Hospital, Jaipur. Out of 196 diagnosed cases, 148 were found to be positive by KOH examination while culture positive cases were 160. Trichophyton rubrum was the most common etiological type reported from 53 cases followed by Trichophyton mentagrophytes (23 cases). Trichophyton verrucosum, Trichophyton ferrugineum, Trichophyton concentricum, Trichophyton megninii, Microsporum canis, Microsporum audouinii, Microsporum fulvum were reported for the first time in Jaipur. Scopulariopsis sp., Paceilomyces sp. and Curvularia sp.were also reported for the first time from human skin in Jaipur. Tinea corporis was the most common clinical type reported from all age groups. Tinea cruris was the second most common clinical type, followed by tinea capitis, tinea manuum, tinea unguium, etc. These infections were observed more frequently in the age group of 21-30 (26 %), followed by 31-40 (18.8%) and 11-20 (16.3%). Males (75.5%) were more infected than females (24.5%).
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