Relevance. The plasma treatment of ready-to-eat foods the potential use to increase a storage time and a food safety. On the first step seems important to evaluate the bactericidal effect of a non-thermal (non-equilibrium) plasma on a natural association bacteria isolated from the food.Methods. The non-thermal plasma source based on an argon plasma jet in a microwave (streamer) discharge at atmospheric pressure was used. Lactobacillus culture isolated from a walnut in the natural association and the vegetative form was treated. Sowing was carried on Petri dishes with Endoʼs agar. The exposure time is 60 s, 180 s and 360 s. The distance from the plasma jet source’s nozzle to the Endoʼs agar surface was 30 mm. The diameter of the growth inhibition zone of Lactobacillus on the dense nutrient medium was determined.Results. The round transparent Endo’s agar area — the zone of growth inactivation of colony-forming units in bacterial lawn of Lactobacillus after exposure to argon plasma jet was registered. After counting colony-forming units, a decrease in the number of Lactobacillus colonies grown after a 360-second exposure was found. At a 60-second exposure, no significant decrease in the number of grown colony-forming units was observed. The bactericidal effect is noticeable after a 180-second exposure to the plasma jet. With an increase in the exposure time to 360 s, the sterilization area on the surface of the agar in the Petri dish increased. The maximum diameter of the Lactobacillus growth inactivation zone did not exceed the diameter of the nozzle of the plasma jet source (36 mm). Under the influence of plasma, the color of the Endo agar changed in the zone of direct exposure to the plasma jet.
The paper presents the vegetation experiments results on the low-temperature argon plasma effect on barley plants ( Hordeum vulgare L.) of the Vladimir cultivar and its influence on morphophysiological parameters and yield. Plasma treatment was once at three organogenesis stages of barley plants: 3rd leaf, tillering and booting. Plasma exposure was 15 and 30 min. The barley plants were grown to full maturity. Analysis of barley yield structure did not reveal clear patterns in the change in most parameters resulted from the plasma treatment. However, 15 min plasma exposure on barley plants in the critical development stage (3rd leaf) increased by 77.8 % (p 0.05) the root weight of plants compared with control. After treatment at the tillering stage, the number of spikelets per main stem ear increased by 18.5 % (p 0.001) after 15 min plasma exposure, and by 11.17 % (p 0.05) after 30 min exposure. An increase in the number of productive stems and the number of grains per lateral stem ear was observed. At the same time, 30 min exposure in the 3rd leaf stage reduced by 7 % (p 0.05) the plant height. And the treatment in the tillering stage reduced by 39 % (p 0.01) the root weight of barley plants. The effect of low-temperature plasma on barley plants at the booting stage was less expressed to the plasma effect at earlier development stages. This can be explained by the lower sensitivity of this stage of organogenesis. The obtained effects of single exposure to low-temperature argon plasma at different organogenesis stages of barley plants can be useful to increase barley yields.
Microbial organisms are key pathogens for plants, animals, and humans. The elimination of pathogenic microbes is an essential topic for researchers. Non-thermal atmospheric plasma (NTAP) can potentially inactivate pathogenic microorganisms. This paper draws the results of the biocidal effect study in pre-treatment of barley seeds by NTAP. The effect was observed in 7-day-old seedlings. The results of reducing the total microbial number (by 36.7% after 5 minutes of exposure) were obtained. Thus, treatment with non-thermal plasma can reduce the microbiological contamination of agricultural plant seeds.
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