An isotypic structure of system antibody response to influenza A(H1N1)pdm09 was analyzed in adult volunteers vaccinated with two inactivated monovalent subunit vaccines against pandemic influenza. The comparison group consisted of patients infected with influenza A(H1N1)pdm09 virus. In vaccinated volunteers the more active response of influenza-specific antibodies both with neutralizing properties (IgG1, IgG2, IgG3) and associated with allergic inflammation (IgG and IgE) was observed in comparison with infected patients. The high activity of the virus-specific serum IgA was observed both in infected patients and vaccinated volunteers. Antiviral hemagglutinating activity of antibodies in post-vaccination sera of vaccinated volunteers, unlike sera obtained from infected patients in the phase of recovery, were higher than protective level (1:40) according to HAI data.
Antiepidemic measures were limited effectiveness for several years Objectives of this research were formulated as an assessment of the immunogenicity activity of the influenza virus A(H1N1)pdm09 in the composition of modern trivaccines Immunogenicity of the influenza virus A(H1N1)pdm09 in the vaccinated by vaccine was assessed by graphing, reflecting the dynamics of the multiplicity growth of antibodies (MG) and medium ratio of antibodies increasing (MR) in sera for several groups vaccinated. For comparison of vaccinated immunity was determined by traditional methods of evaluation of the immune response. As a result of the research, differences in the immunogenicity activity of the virus were revealed, which are reflected in antibody titers and the multiplicities of their growth from 2 to 4 - 8 times with the applying of similar quality vaccines. These changes couldn’t be observed with accounting study of the immune response. When immunized with a vaccine with an antigen dose of 5 mg HA identified a group of«silent» volunteers (8%) who did not respond to promotion with antigen A(H1N1)pdm09. Increasing dose of the influenza virus A(H1N1)pdm09 to 15 mg/dose in the split vaccine were result of the elimination of the group of«silent» volunteers. Simultaneously was observed a significant increase in the immune response in serum titers (up to 32-fold) and antibody growth rates Accordingly, using of the graphical form of accounting made it possible to better assess the details of the formation of collective immunity to the virus A(H1N1)pdm09 and the nature of its deviations in a number of cases.
Currently, the assessment of the immunogenic properties of influenza viruses as a part of influenza vaccines, is carried out by using seroprotection, seroconversion as well as the rate of increases in post-vaccination antibodies. At the same time, significant differences in the immunogenicity of vaccines related to dynamic formation of high antibody titers responsible for long-term protection of the vaccinated, are neglected.Influenza viruses such as A (H1N1) pdm09 that caused 2009-2010 pandemic continue to circulate in the population, therefore, the assessment of the immunogenic activity of vaccine viruses prepared during the pandemic period is interesting in for the methodology to prepare pandemic vaccines to be used in various groups (adults, children, elderly people).Analyzing immunogenicity of influenza vaccines used during the 2009-10 swine influenza pandemic and the post-pandemic period up to the year 2014 was carried out by applying the graphical method for assessing immunogenicity (immunographs) measured as follows: for each group of vaccinated subjects (depending on the vaccine used), an increased rate in antibody level was calculated and the graphs of immunogenicity were plotted. An increased rate of serum antibodies magnitude from vaccinated subjects and the number of sera (in%) with a given fold increase rate in antibody level from 1 to the maximum magnitude were plotted on the x- and y-axis, respectively. The proposed method for assessing immunogenicity allows to plot immunogenicity graphs regardless of the serum antibodies level found in volunteers. The assessment described above revealed a several features for developing immune response to the pandemic virus A (H1N1)pdm09 such as the lack of immune response in a substantial number of adult volunteers (25-27%%) and young children (60-70%%) after monovaccine administration. The reason for such immune response can be both an insufficient dose of vaccine-containing viral antigen and suppressed immune response caused by the influenza A(H1N1)pdm09.A study on the immunogenic properties for seasonal influenza vaccines containing the influenza A (H1N1) pdm09 virus antigen in the years 2010 - 2014 revealed a variety in emerging humoral immunity ranging from a short-term, low-frequency increase in antibodies from vaccinated children to the formation of high antibody titers in elderly.Practically, immunographic analysis of influenza vaccines particularly those derived from the influenza A (H1N1)pdm09 virus, may result in proposing recommendations to increase an antigenic load at the beginning of a pandemic cycle and/or block the suppressive properties of vaccine-contained viruses in pediatric vaccines, because escalating virus dose in the vaccine may not always be achievable in this case.
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