ABSTRACT:Halophyte plants are known to tolerate environments with high salt concentrations, although there are intraspecific differences in the level of tolerance borne by these plants. Portulaca oleracea L., commonly known as Purslane, is mentioned in the literature as a halophyte species that shows tolerance to salinity, but this tolerance is genotype-dependent.The present study aimed to evaluate if a Purslane access (B1) is highly tolerant to salinity, to be used in future studies on the mechanism(s) promoting this tolerance. For that, morphological changes the B1 access were evaluated once submitting it to saline stress at concentrations of 0.0; 0.2; 0.4; 0.6; 0.8; 1.0; 1,2; 1.4; 1.6; 1.8 and 2.0 g of NaCl / 100 g of substrate. Seeds were germinated in culture medium (MS ½ strength, Phytagel 0.2%, and pH 5.8) and then transferred to 200 mL plastic cups with 100 g of substrate. After 35 days, saline solutions containing different amounts of salts were added to the substrate in order to subject the plants to the different levels of salts above mentioned. The experiment was conducted in a plant growth chamber in which temperature was set at 25 ±2°C, 65 ±5% of air relative humidity and 500±20 μmol/m -2 /s -1 of PAR, and a photoperiod of 16/8 h (light/dark). The most common morphological characteristics (shoot growth, leaf area and number, stem succulence, leaf color and stem color ) were monitored for a period of up to 12 days, after the onset of saline stress.The experimental design was completely randomized with three replicates per treatment.Purslane plants showed practically no changes in the variables evaluated up to 1.2 g of salt.Even in the highest concentrations, the plants remained alive. KEYWORDS:Abiotic stress, salt tolerance, Purslane. ALTERAÇÕES MORFOLÓGICAS EM PALAVRAS-CHAVE:Estresse abiótico, tolerância à salinidade, Beldroega.
Soil salinity is one of the main abiotic stresses limiting growth and productivity of plants. Salinity tolerance can be achieved by heterologous expression or gene editing strategies. Setaria viridis has been used as model plant in fast proof-of-concept studies aiming gene function validation. To use a plant as a model for studying a particular type of stress, one must show that it is susceptible (not tolerant) to that stress. Here we aimed to perform physiological analyses to prove that access A 10.1 of S. viridis can be used for the validation of genes for salt tolerance. showed reduction in the plants biomass which was directly proportional to the increase in the salt amount in the substrate up to 6 g/dm³. The plants submitted to salt contents above 8 g of NaCl did not survived to stress. All salt stressed plants showed a reduction in the rates of stomatal conductance and transpiration. However, only plants submitted to 6 g NaCl had a reduction in the net assimilation rate of CO2 and in the chlorophyll content.KEYWORDS: Salinity, tolerance, Setaria.
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