HighlightsEMS and UV mutagenesis of Nannochloropsis salina combined with FACS for mutant enrichment.Productivity of EMS mutants increased by 76% and showed range of FA profile changes.Dual EMS and UV mutants accumulated 3 fold more lipid than the wild type.Elevation in lipid content comes with a cost to growth rate impacting productivity.Mutants suitable for divergent industries generated (biofuel, high value PUFA production).
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