Isolation and characterization of novelten markers were polymorphic, exhibiting an allelic number (N A ) from 2 to 6 alleles per locus. The effective number of alleles (N E ) ranged from 1.60 to 3.08 (average of 2.30). The observed (H O ) and expected (H E ) heterozygosities ranged from 0.13 to 0.67 (average of 0.39) and 0.29 to 0.63 (average of 0.50), respectively. Linkage disequilibrium was not significantly detected for any pair of loci, and only two loci (BettaMS23 and BettaMS28) showed significant deviations from Hardy-Weinberg expectations. Of these, six loci could be amplified in genomic DNA of the closely related species B. imbellis and three loci in B. smaragdina. These microsatellite markers could be used as a tool to investigate genetic diversity and population structure, as well as breeding programs in hatcheries.