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The chronological development of destructive and nondestructive methods designed to determine the glucose concentration in plasma, serum, and whole blood is presented in the review. Destructive procedures are divided into spectrophotometric, spectrofluorimetric, diffraction, amper ometric, potentiometric, conductometric, chromatographic, gas chromatographic-mass spectromet ric, titrimetric, and calorimetric types, as well as reflectance photometry. A brief overview of these methods and the results of comparative tests are given; the procedures are systematized. It is noted that certain types of enzymatic spectrophotometric and amperometric methods have received wide accep tance for analyzes in clinical diagnostic laboratories. Outside clinical laboratories, enzymatic amper ometric and spectrophotometric procedures and reflectance photometry procedures using test strips are widely used. Chromatography-mass spectrometry procedures are often applied to certify standard serum samples. Spectrofluorimetric, diffraction, and chromatographic techniques are common in sci entific biomedical research. Nondestructive methods like nonenzymatic spectrophotometric, nonen zymatic amperometric, potentiometric, conductometric, titrimetric, and calorimetric procedures, as well as a number of enzymatic spectrophotometric procedures, in most cases, are not used in modern clinical and diagnostic practice.