The transcription factor nuclear factor-kappaB (NF-kappaB) is a regulator related to cellular inflammation, immune responses and carcinogenesis. Therefore, components of the NF-kappaB-activating singnaling pathways are frequent targets for the anti-inflammatory and anticancer agents. In this study, CYL-19 s and CYL-26z, two synthetic alpha-methylene-gamma-butyrolactone derivatives, were shown to inhibit the tumor necrosis factor-alpha (TNF-alpha)-induced intercellular adhesion molecule-1 (ICAM-1) expression in human A549 alveolar epithelial cells and the adhesion of U937 cells to these cells. RT-PCR analysis also demonstrated their inhibitory effects on TNF-alpha-induced ICAM-1 mRNA expression. TNF-alpha-induced ICAM-1 and NF-kappaB-dependent promoter activities were attenuated by CYL-19 s and CYL-26z. ICAM-1 promoter activities induced by the over-expression of wild-type NF-kappaB-inducing kinase and IkappaB kinase beta (IKKbeta) were also inhibited by both compounds. Furthermore, CYL-19 s and CYL-26z inhibited the TNF-alpha-induced phosphorylation and degradation of IkappaBalpha and NF-kappaB-specific DNA-protein binding activity via targeting IKK complex directly, without any effect on the activations of other kinases such as ERK1/2 and p38. In addition to ICAM-1 expression, CYL-19 s and CYL-26z also suppressed other NF-kappaB-mediated gene expressions such as matrix metalloproteinase-9 (MMP-9) mRNA and cyclooxygnease-2 (COX-2) protein. In Matrigel assays, ICAM-1 and COX-2 expressions induced by TNF-alpha elicited A549 and NCI-H292 cell invasion, respectively, and these effects were inhibited by both compounds. In summary, our data demonstrated that CYL-19 s and CYL-26z down-regulate the TNF-alpha-induced inflammatory genes expression through suppression of IKK activity and NF-kappaB activation. These agents may be effective in the anti-inflammatory and anticancer therapy.
This paper presents a real-time heart-rate estimator using the ECG data which are received via wireless bluetooth receiver and measured with wearable electrodes made of steel textile in a wearing system. The measured ECG signals, because of the movable electrodes and the resulting unfixed contacts, demonstrate high baseline wandering phenomena and high noisy aberrations especially when the wearing person is in motion, making the heart-rate estimation a sophisticated work. To conquer this problem, the presented heartrate estimator first uses the subspace technique to remove the baseline wandering in ECG signals, and then applies the adaptive notch filter (ANF) technique to obtain the heartrate estimate. Experiments for ECG data from the person with initially sitting still through walking steadily to jogging, demonstrate that the presented method obtains heartrate estimates which satisfactorily reflect the motion status of the person. The vital system embedded with the capability of real-time heart-rate estimation makes it highly suitable for applications of remote healthcare and wellness.
A system with wearable sensors for detecting and sensing vital and physical motion signals such as the ECG, respiration, temperature, sweatiness, three-axis accelerations, and the motions of both knee ankles and elbows is presented. The system is also equipped with a bluetooth transceiver such that the obtained sensor signals can be transmitted to mobile phones or remote computers for either monitoring or post signal processing to extract knowledges or information. The presented system is embedded with signal processing algorithms such as the heart rate, the respiratory period, the body temperature, the degree of sweatiness, and the posture of wearing person are demonstrated. The heart rate and heart rate variation from the measured ECG data are estimated using an adaptive ESPRIT algorithm. This system is highly suitable for applications of remote healthcare and wellness.
In this study, we propose a wearing system with four sensors, ECG (electrocardiogram), three-axis accelerometer, temperature, and tight-switch, applied for remote monitoring system in home-care. The sensors ECG, measured with wearable electrodes made of the steel textile to generate the real-time heart-rate estimator, tight-switch, made of the steel textile to check whether wearing person dresses properly, accelerometer, and temperature parameters are received via the ZigBee receiver within an exquisite belt. Since the movable textile electrodes will cause of unfixed contacts when the wearing person is in motion, making the heart-rate estimation much a sophisticated work, the tight-switch sensor and FIR (Filter Impulse Response) filter technology are applied here to get the more satisfiable heart-rate. The other two bio-sensors can detect the whether fall-down or not and normal body-temperature of this wearing person. Moreover, the ZigBee device with small size, low-power consumption, and high-reliability characteristics is designed to transmit the detected bio-information from these four sensors. Therefore, the vital system embedded with the capability of real-time heart-rate estimation and transmission makes it highly suitable for applications of remote healthcare and wellness.
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