Abstract. Utilizing the mechanism of HOCl-mediated cyclization of rhodamine 101 thiosemicarbazide (non-fluorescent) to rhodamine 101 oxadiazoles (fluorescent), we successfully developed a novel off-on far-red fluorescent probe ( 1 ) toward HOCl with high selectivity, great functionality at physiological pH and good cell-membrane permeability. Probe 1 was linearly proportional to the concentration of HOCl in the range of 1-10 M and the detection limit is 0.3 M. Moreover, the proposed probe (1) was successfully used for the detection of hypochlorous acid in HepG2 cells by fluorescent imaging.
Abstract. A novel rhodamine 101 derivative has been synthesized and employed as a fluorescent off-on probe for monitoring Cu 2+ in aqueous solutions and living cells. Under physiological conditions, it exhibits a remarkable increase in absorbance at 587 nm and an enhanced fluorescence at 615 nm toward Cu 2+ in a highly sensitive, selective and rapid manner. The probe can be applied to the quantification of Cu 2+ with a linear concentration range covering from 0.4 M to 16 M (R 2 = 0.9955) and a detection limit of 40 nM. Furthermore, the proposed probe has been successfully applied for fluorescence imaging of Cu 2+ in living cells.
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