The seminal importance of DNA sequencing to the life sciences, biotechnology and medicine has driven the search for more scalable and lower-cost solutions. Here we describe a DNA sequencing technology in which scalable, low-cost semiconductor manufacturing techniques are used to make an integrated circuit able to directly perform non-optical DNA sequencing of genomes. Sequence data are obtained by directly sensing the ions produced by template-directed DNA polymerase synthesis using all-natural nucleotides on this massively parallel semiconductor-sensing device or ion chip. The ion chip contains ion-sensitive, field-effect transistor-based sensors in perfect register with 1.2 million wells, which provide confinement and allow parallel, simultaneous detection of independent sequencing reactions. Use of the most widely used technology for constructing integrated circuits, the complementary metal-oxide semiconductor (CMOS) process, allows for low-cost, large-scale production and scaling of the device to higher densities and larger array sizes. We show the performance of the system by sequencing three bacterial genomes, its robustness and scalability by producing ion chips with up to 10 times as many sensors and sequencing a human genome.DNA sequencing and, more recently, massively parallel DNA sequencing 1-4 has had a profound impact on research and medicine. The reductions in cost and time for generating DNA sequence have resulted in a range of new sequencing applications in cancer 5,6 , human genetics 7 , infectious diseases 8 and the study of personal genomes 9-11 , as well as in fields as diverse as ecology 12,13 and the study of ancient DNA 14,15 . Although de novo sequencing costs have dropped substantially, there is a desire to continue to drop the cost of sequencing at an exponential rate consistent with the semiconductor industry's Moore's Law 16 as well as to provide lower cost, faster and more portable devices. This has been operationalized by the desire to reach the $1,000 genome 17 .To date, DNA sequencing has been limited by its requirement for imaging technology, electromagnetic intermediates (either X-rays 18 , or light 19 ) and specialized nucleotides or other reagents 20 . To overcome these limitations and further democratize the practice of sequencing, a paradigm shift based on non-optical sequencing on newly developed integrated circuits was pursued. Owing to its scalability and its low power requirement, CMOS processes are dominant in modern integrated circuit manufacturing 21 . The ubiquitous nature of computers, digital cameras and mobile phones has been made possible by the low-cost production of integrated circuits in CMOS.Leveraging advances in the imaging field-which has produced large, fast arrays for photonic imaging 22 -we sought a suitable electronic sensor for the construction of an integrated circuit to detect the hydrogen ions that would be released by DNA polymerase 23 during sequencing by synthesis, as opposed to a sensor designed for the detection of photons. Although a variety ...

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Studies of the proteome would benefit greatly from methods to directly sequence and digitally quantify proteins and detect posttranslational modifications with single-molecule sensitivity. Here, we demonstrate single-molecule protein sequencing using a dynamic approach in which single peptides are probed in real time by a mixture of dye-labeled N-terminal amino acid recognizers and simultaneously cleaved by aminopeptidases. We annotate amino acids and identify the peptide sequence by measuring fluorescence intensity, lifetime, and binding kinetics on an integrated semiconductor chip. Our results demonstrate the kinetic principles that allow recognizers to identify multiple amino acids in an information-rich manner that enables discrimination of single amino acid substitutions and posttranslational modifications. With further development, we anticipate that this approach will offer a sensitive, scalable, and accessible platform for single-molecule proteomic studies and applications.

Perturbed-angular-correlation (PAC) spectroscopy was used to measure nuclear-electric-quadrupole interactions in the orthorhombically-distorted perovskites SrRuO, and CaRuO, over temperatures ranging from laboratory to very high temperatures. At 77 K, PAC spectroscopy was used to measure combined electric-quadrupole and magnetic-dipole interactions in magnetically ordered SrRu03 and pure electric-quadrupole interactions in paramagnetic CaRu03. The "'In~"'Cd PAC probe was used for these measurements, and it substituted into the Ru site in SrRu03 and very likely into the Ru site in CaRu03. The temperature dependence of the electric-field-gradient (EFG) parameters for SrRu03 indicates the onset of a structural phase transition at approximately 800 K. The presence of this transition indicates that the laboratory-temperature structure of SrRu03 has lower-than-cubic symmetry. At very high temperatures) 1600 K, the structure of CaRu03, as given by the EFG parameters, becomes very similar to the laboratory-temperature structure of SrRu03. At 77 K in SrRu03, the measured Ru-site supertransferred hyperfine field is 39+3 kOe. Using "Ru and "Fe Mossbauer-effect information and other ' "In~"'Cd PAC measurements, the magnetic hyperfine fields at the Ru site in SrRu03 and at the Fe site in PrFe03 are compared. 4.2 K SR is magnetically ordered and CR is not. Thus the initial report of antiferromagnetism in CR is incorrect. Both the high-temperature crystal structures of SR and CR and the low-temperature magnetic properties, pri