The use of microalgae biomass as a suitable alternative feedstock for biofuel production has been promoted in the field of green biotechnology. In this present study, the microalgae were isolated from freshwater samples. The predominant strain was screened from the samples and grown in four different growth media, including modified Bold's Basal Medium (BBM), modified CFTRI medium, BG11 medium and CHU medium, to find the suitable growth medium to enrich biomass production. In total three microalgae colonies were identified based on their colony morphology microscopically by using a light microscope. The predominant strain was confirmed as Asterarcys quadricellulare using 18S rRNA sequencing. The growth of microalgae was investigated based on parameters like dry weight, pigment composition such as chlorophyll a, chlorophyll b, carotenoid and lipid content in the microalgae. Among the four different media, modified BBM medium showed maximum dry weight (1.44 ± 0.015 g/L), chlorophyll a (23.07 ± 0.049 mg/L), chlorophyll b (16.76 ± 0.010 mg/L), carotenoid (8.92 ± 0.031 mg/L) and lipid content (375 ± 0.020 mg/L) on the 25th day of culture. The gas chromatography mass spectrometry (GC/MS) analysis showed the presence of major fatty acids stearic acid, palmitic acid and oleyl alcohol in the microalgae. Therefore the high lipid content and fatty acid profiles of Asterarcys quadricellulare are becoming a promising suitable strain for biofuel production with modified BBM medium.
Polylactic acid (PLA) is an environmentally friendly, biodegradable, and lowcost thermoplastic material suitable for 3D printing. Complex shapes can be easily produced and hence PLA has been used for many applications. This study presents the damping characteristics of two robotic gripper fingers. The first gripper is composed of a PLA surface layer and a magnetorheological (MR) fluid core and the second gripper is composed of a PLA surface layer bonded to a surface of unimorph piezoelectric Macro Fiber Composite (MFC).The magnetic field is applied by using permanent magnets while the magnetic field strength is varied by changing the distance between the permanent magnets. The electric field is applied by using a micro-amplifier and regulated using data acquisition systems. The natural frequency and damping ratio of the PLA gripper finger are determined by free vibration analysis using LabVIEW software. The damping ratio and vibration amplitude suppression for the PLA grippers was increased as the magnetic field strength changed from 0G to 600G (MR) and as the electric field changed from 0V to 360V (MFC).
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