This study examined a new approach for starting up a bioelectrochemical system (BES) for oxalate removal from an alkaline (pH > 12) and saline (NaCl 25 g/L) liquor. An oxalotrophic biofilm pre-grown aerobically onto granular graphite carriers was used directly as both the microbial inoculum and the BES anode. At anode potential of +200 mV (Ag/AgCl) the biofilm readily switched from using oxygen to graphite as sole electron acceptor for oxalate oxidation. BES performance was characterised at various hydraulic retention times (HRTs, 3-24 h), anode potentials (-600 to +200 mV vs. Ag/AgCl) and influent oxalate (25 mM) to acetate (0-30 mM) ratios. Maximum current density recorded was 363 A/m at 3 h HRT with a high coulombic efficiency (CE) of 70%. The biofilm could concurrently degrade acetate and oxalate (CE 80%) without apparent preference towards acetate. Pyro-sequencing analysis revealed that known oxalate degraders Oxalobacteraceae became abundant signifying their role in this novel bioprocess.
Destruction of oxalate from alumina-refining process liquor is considered essential for many alumina refineries around the world. Some refineries have embraced the use of aerobic bioreactors as a cost-effective destruction method. These processes are often supplemented with an external nitrogen (N) source to facilitate microbial activity, even though such augmentations are undesirable due to increase of operational costs. Until now, there has also only been little information on oxalate degradation kinetics, although this knowledge is essential to design bioreactor processes. Hence, this study aimed at determining oxalate degradation kinetics in two aerobic packed bed biofilm reactors under both N-supplemented and N-deficient conditions. Michaelis-Menten equation was used to derive kinetic parameters for specific oxalate degradation. The N-deficient culture had a higher affinity (K m of 458.4 vs. 541.9 mg/L) towards oxalate and a higher maximum specific oxalate removal rate (V max of 161.3 vs. 133.3 mg/(h•g biomass)) compared to the N-supplemented culture, suggesting that the N-deficient culture is better suited to remove oxalate. Microbial community analysis also showed differences in the composition of the two cultures. Based on the kinetic parameters derived, a novel two step oxalate removal process was proposed that capitalises on higher specific oxalate removal rates for efficient oxalate destruction from waste streams of alumina industry.
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