For the first time, Stagonosporopsis caricae is reported causing leaf spots on the non-conventional fruit crop Vasconcellea monoica (Caricaceae). The fungus was identified in Brazil based on morphological and molecular features and its pathogenicity was demonstrated. This is the first pathogen to be reported on V. monoica.
During surveys conducted in South America and Africa to identify natural fungal enemies of coffee leaf rust (CLR), Hemileia vastatrix, over 1500 strains were isolated, either as endophytes from healthy tissues of Coffea species or as mycoparasites growing on rust pustules. Based on morphological data, eight isolates—three isolated from wild or semiwild coffee and five from Hemileia species on coffee, all from Africa—were provisionally assigned to the genus Clonostachys. A polyphasic study of their morphological, cultural and molecular characteristics—including the Tef1 (translation elongation factor 1 alpha), RPB1 (largest subunit of RNA polymerase II), TUB (β-tubulin) and ACL1 (ATP citrate lyase) regions—confirmed these isolates as belonging to three species of the genus Clonostachys: namely C. byssicola, C. rhizophaga and C. rosea f. rosea. Preliminary assays were also conducted to test the potential of the Clonostachys isolates to reduce CLR severity on coffee under greenhouse conditions. Foliar and soil applications indicated that seven of the isolates had a significant effect (p < 0.05) in reducing CLR severity. In parallel, in vitro tests that involved conidia suspensions of each of the isolates together with urediniospores of H. vastatrix resulted in high levels of inhibition of urediniospore germination. All eight isolates showed their ability to establish as endophytes in C. arabica during this study, and some proved to be mycoparasites of H. vastatrix. In addition to reporting the first records of Clonostachys associated with healthy coffee tissues and with Hemileia rusts of coffee, this work provides the first evidence that Clonostachys isolates have potential as biological control agents against CLR.
Digitodesmium is a genus of saprobic fungi, generally associated with decaying wood in freshwater habitats or in the soil. As morphologic markers they produce cheiroid, euseptate conidia on sporodochia. During an exam of a necrotic robusta coffee stem sent from Nova Venécia, state of Espírito Santo, to the Plant Clinic at the Universidade Federal de Viçosa (Brazil), for disease diagnosis a fungus, recognized as having the typical features of Digitodesmium was observed. The fungus was isolated in pure culture and DNA was extracted. Sequences of the partial 18S ribosomal RNA gene, large subunit of the nrDNA, internal transcribed spacer and translation elongation factor 1-α were generated. The combination of results of the phylogenetic analysis with the exam of the morphology led to the conclusion that the fungus from coffee stem morphological data showed that this fungus represents a monophyletic distinct lineage within Digitodesmium and an undescribed species for the genus. The concatenate tree also revealed that Digitodesmium is divided in two distinct clades. The novel species can be differentiated morphologically from other species of Digitodesmium by the size of the conidia, the number of arms and the presence of appendages. The new species Digitodesmium polybrachiatum is hence proposed herein. A comparative table of conidial morphology for the species in the genus is also included.
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