Objectives: Psorospermum febrifugum Spach. (Clusiaceae) is a medicinal plant found in Africa. It root bark was used in Benin to treat anaemia. This work aimed to study the therapeutic efficacy of that root bark extract on anaemia treatment. Methodology and Results: Wistar Rats were anaemic by intraperitoneal injection of phenylhydrazine chloridrate. The rats were then fed either with Psorospermum febrifugum Spach root bark extract at 200 or 300 mg / kg weight / day, or with vitafer® as the reference drug. Non-anaemic rats served as controls. The blood count and osmotic resistance of red blood cells carried out on blood samples of rats at different days (D0, D2, D7, J10 and D15) showed a correction of anaemia with the extract in two weeks. The extract screening revealed saponosides, reducing sugars, polyphenols, flavonoids, tannins, proteins, anthocyanins and alkaloids. Conclusion and application of results:The extract completely corrected anaemia in two weeks by increased stimulation of hemoglobin synthesis and the production of young red blood cells released into the blood. Its effect seems dose-dependent and at the 300 mg / Kg dose was even more effective than the vitafer. This remarkable efficiency would certainly be linked to its chemical composition, whose elements would act in an isolated or synergistic way to stimulate haematopoiesis.. In addition, the extract had no effect on blood thrombocytes suggesting a certain specificity of action on the erythrocyte line. Psorospermum febrifugum Spach root bark had an excellent therapeutic efficacy and could be a good candidate for transformation into improved traditional medicines (ITM) for anaemia treatment, after acute and chronic toxicity tests and appropriate clinical trials.
Sickle cell disease was a very common hemoglobinosis in tropical countries. Several plants were used in traditional Beninese medicine to relieve sickle cell crises. The present work was carried out to test the efficiency of Uvaria chamea roots aqueous extract in the treatment of sickle cell crises. Methods: Uvaria chamea roots aqueous extract was preincubated at different concentrations with blood cells from SS red blood cells before or after the Emmel Test. Intra-erythrocyte oxidative stress was measured by methaemoglobin assay after incubation of the extract with hemoglobin. In vivo, the action of the extract on Hemoglobin level, Mean Corpuscular Volume, and platelet count was measured in Wistar rats. Results: The extract significantly prevented the formation of sickle cells at doses of 40 and 20 mg / ml of blood (P <0.05) and significantly reduced sickle cells to the normal biconcave form at the dose of 40 mg / ml (P <0.05). It lowered the production of methemoglobin at a dose of 10 mg / ml, suggesting antioxidant activity. Hemoglobin level, Mean Corpuscular Volume, and platelet count did not significantly increase in treated rats, indicating that the extract did not stimulate erythropoiesis or thrombopoiesis. Conclusion: Uvaria chamea extract inhibited dose-dependent sickling of red blood cells. It reduced intraerythrocyte oxidative stress but did not show hematopoietic activity. It could therefore be considered in the preventive or curative management of sickle cell crises, but not against anemia.
Fruit juices are one of the favorite drinks for children these days. As a result, its production must be organized in such a way as to ensure quality and guarantee the safety of consumers in general and schoolchildren in particular. The objective of this study is to analyze the health quality of bissap, lemon and pineapple juices sold in some public schools in the district of Agblangandan. For this, we collected from the vendors, 12 pineapple juice, 14 bissap juice and 8 lemon juice during the morning recess. Then, the microbiological and physico-chemical analyzes were carried out on the juices. The microbiological analyzes made it possible to identify the presence of total mesophilic flora (1.09.104 CFU/ml), total Coliforms (2.78.103 CFU/ml), Escherichia coli (4.44.102 CFU/ml), Enterococci (86.1 CFU/ml) and Clostridium (13.2 CFU/ml) in bissap juices. These same germs have been identified in lemon and pineapple juices with varying loads. As for the physico-chemical parameters, the results revealed a high rate of turbidity, conductivity, temperature and pH of the juices. Factors such as failure to master hygiene rules when bagging juices, non-compliance with hygiene rules during handling and storage and then inappropriate hygiene practices are origin of the contamination of these juices. It is therefore urgent to prioritize the hygiene and health of school children through awareness-raising, education and regular and rigorous health control of the juices sold by sworn agents.
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