Blood and the linings of blood vessels may be regarded as a fifth tissue type. The human body contains 5 × 10 9 red blood cells (RBCs) per ml, a total of 2.5 × 10 13 cells in the 5 l of blood present in the body. With an average lifetime of 125 days, human RBCs are destroyed by leukocytes in the spleen and liver. Nowadays red blood cells are extensively used to study various metabolic functions. Nanoparticles (NP) are being widely accepted for drug delivery system. This review summarizes the red blood cells, NPs and their characteristics on the basis of the RBC components along with drug delivery systems through RBCs. Further, we also discussed that how erythrocytes can be used as an efficient in vitro model for evaluating the efficacy of various nanocomposite materials.
Chronic obstructive pulmonary disease accounts as the leading cause of mortality worldwide prominently affected by genetic and environmental factors. The disease is characterized by persistent coughing, breathlessness airways inflammation followed by a decrease in forced expiratory volume1 and exacerbations, which affect the quality of life. Determination of genetic, epigenetic, and oxidant biomarkers to evaluate the progression of disease has proved complicated and challenging. Approaches including exome sequencing, genome‐wide association studies, linkage studies, and inheritance and segregation studies played a crucial role in the identification of genes, their pathways and variation in genes. This review highlights multiple approaches for biomarker and gene identification, which can be used for differential diagnosis along with the genome editing tools to study genes associated with the development of disease and models their function. Further, we have discussed the approaches to rectify the abnormal gene functioning of respiratory tissues and various novel gene editing techniques like Zinc finger nucleases (ZFN), transcription activator‐like effector nucleases (TALEN), and clustered regulatory interspaced short palindromic repeats/CRISPR‐associated protein 9 (CRISPR/Cas9).
:
Regardless of advances in detection and treatment, breast cancer is a major cause of women
death and affecting about 1.5 million women all over the world. Since the last decade, genome
wide association studies (GWAS) have been extensively conducted for breast cancer to define
the role of miRNA as a tool for diagnosis, prognosis and therapeutics. MicroRNAs are small,
non-coding RNAs that are associated with regulation of key cellular processes such as cell
multiplication, differentiation, and death. They cause disturbance in the cell physiology by
interfering directly with the translation and stability of a targeted gene transcript. MicroRNAs
(miRNAs) constitute large family of non-coding RNAs which regulate target gene expression
and protein levels that affect several human diseases and are suggested as the novel markers or
therapeutic targets, including breast cancer. MicroRNA (miRNA) alterations are not only
associated with metastasis, tumor genesis but also used as biomarkers for breast cancer
diagnosis or prognosis. These are explained in detail in the following review. This review will
also provide an impetus for more studies on the role of microRNAs in breast cancer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.