Tomato spotted wilt virus (TSWV) causes significant economic losses in the commercial culture of tomato (Lycopersicon esculentum Mill .) . Culture practices and introgression of natural sources of resistance to TSWV have only been marginally effective in controlling the TSWV disease. Recently however, high levels of protection against TSWV have been obtained by transforming tobacco with a chimaeric gene cassette comprising the TSWV nucleoprotein gene . This report demonstrates the successful application of this newly-created TSWV resistance gene in cultivated tomato . Transformation of an inbred tomato line with the TSWV nucleoprotein gene cassette resulted in high levels of resistance to TSWV that were maintained in hybrids derived from the parental tomato line. Therefore, transformant lines carrying the synthetic TSWV resistance gene make suitable progenitors for TSWV resistance to be incorporated into the breeding programmes of tomato .
Cucumber mosaic virus (CMV) infections rank among the most devastating diseases in the commercial culture of tomato (Lycopersicon esculentum Mill .), for which suitable sources of natural resistance are not available . The concept of pathogen-derived resistance, however, offers an alternate approach to combat plant viral diseases by transformation of crops with nucleotide sequences derived from the viral genome . This report demonstrates the successful application of such a pathogen-derived resistance gene comprising the CMV coat protein (CP) gene, to generate protection to CMV infections in cultivated tomato . Transformation of an inbred tomato line with the CMV CP gene isolated from a subgroup I strain, engendered high levels of protection to various CMV strains, including a virulent strain causing lethal necrosis and a typical subgroup II strain . Moreover, when challenged by natural infection through aphid vectors in open field, levels of protection were largely maintained in hemizygous hybrids . In all, these results demonstrate that synthetic resistance genes based on the CMV CP gene make excellent sources of broad spectrum resistance to CMV infections for introgression into tomato breeding programs .
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