A new propionate (prp) locus of S. typhimurium was defined by mutation, was located to minute 8 of the chromosome, and was shown to be transcribed in the clockwise direction. A plasmid carrying the wild-type prp+ locus was isolated by complementation and its initial physical characterization is presented. Transcriptional regulation of prp was studied using MudI1734(lacZ+) operon fusions. Propionate stimulated prp transcription in a merodiploid strain containing prp+ and a prp::MudI1734 fusion, but failed to stimulate transcription of the same fusion in a haploid genetic background. prp transcription was reduced by a factor of 2 in strains deficient in the synthesis of the global regulatory protein FruR; fruR mutants failed to grow on propionate. Propionate blocked growth of prp mutants on medium containing succinate as carbon/energy source.
A new propionate (prp) locus of S. typhimurium was defined by mutation, was located to minute 8 of the chromosome, and was shown to be transcribed in the clockwise direction. A plasmid carrying the wild-type prp+ locus was isolated by complementation and its initial physical characterization is presented. Transcriptional regulation of prp was studied using MudI1734(lacZ+) operon fusions. Propionate stimulated prp transcription in a merodiploid strain containing prp+ and a prp::MudI1734 fusion, but failed to stimulate transcription of the same fusion in a haploid genetic background. prp transcription was reduced by a factor of 2 in strains deficient in the synthesis of the global regulatory protein FruR; fruR mutants failed to grow on propionate. Propionate blocked growth of prp mutants on medium containing succinate as carbon/energy source.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.