A liquid chromatography method was developed and validated for the determination of gemifloxacin in human plasma using chloramphenicol as internal standard to achieve lower quantification limit. Acetonitrile was used to precipitated and extracted analyte and internal standard from plasma by Protein Precipitation. Analysis was performed isocratically on C 18 column using 25% acetonitrile and 75% 0.02 M phosphate buffer as mobile phase. The method was demonstrated to be linear from 0.003 µg/mL to 5 µg/mL with the lower limit of quantitation of 0.003 µg/mL. The method was successfully applied for the bioequivalence study of gemifloxacin after a single oral administration of 320 mg gemifloxacin mesylate tablets to 12 healthy volunteers.
Lead is environmental pollutant that even in low trace is harmful for human health and wildlife. In this study we presented a colorimetric method for determination of Pb 2+ in water by aggregation of silver nanoparticles capped citrate in the presence of deferoxamine as chelating agent. Silver nanoparticles were prepared by reduction of AgNO 3 with NaBH 4. In the presence of Pb 2+ , mixture of silver nanoparticles capped citrate and deferoxamine aggregated and color of solution changed from light yellow to violet that depends on Pb 2+ concentration. As a result of aggregation, local surface plasmon resonance (LSPR) band of silver nanoparticles around 440 nm decreases and a new band appears at 580 nm that depend on Pb 2+ concentration. Under optimized conditions, linear relation of concentration of Pb 2+ and absorbance ratio (A 580 /A 440), was from 0.19 to 1.29 µmol L-1 and limit of detection of 0.056 µmol L-1 was found. Control experiments with the addition of 10 other metal ions
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