Perennial rye grass is a widely used forage species in Ireland, on which the ruminant sector of agriculture is heavily dependent. While this species of grass is the primary source of fodder for cows, it is also abundant in plant protein, which could form a potential alternative ingredient in monogastric animal feed using a green biorefinery approach. In this study, perennial rye grass was processed using a novel biorefining process to extract value added products including protein as a potential replacement for soybean meal in monogastric feeds. Feed trials were conducted on a commercial farm with 55 weaner pigs for 31 days until slaughter. The diets comprised a control and a trial diet which integrated the green biorefinery protein concentrate. The effects of the new diet were determined by measuring the daily feed intake (DFI), average weight gain (AWG) and feed conversion ratio (FCR). Amino acid profiles of grass protein concentrate and soybean meal were comparable, with the latter having a slightly higher amount of total protein content, lysine and cysteine. The DFI and ADW indicated that the treatment diet was superior to the control. DFI for the treatment diet (1.512 kg/d) was 8% higher than the control diet (1.400 kg/d) by the end of the trial. Additionally, the ADW for the treatment diet was 6.44% higher than that achieved in the control sample. Meanwhile, FCR calculations indicated that the treatment diet is just as efficient as the conventional diet. Overall, the results of the study indicate positive potential for perennial ryegrass-derived green biorefinery protein concentrate as an alternative protein source for pig feed formulations in Ireland.

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Global warming and climate change are imminent threats to the future of humankind. A shift from the current reliance on fossil fuels to renewable energy is key to mitigating the impacts of climate change. Biological raw materials and residues can play a key role in this transition through technologies such as anaerobic digestion. However, biological raw materials must also meet other existing food, feed and material needs. Green biorefinery is an innovative concept in which green biomass, such as grass, is processed to obtain a variety of protein products, value-added co-products and renewable energy, helping to meet many needs from a single source. In this study, an analysis has been conducted to understand the renewable energy potential of green biorefinery by-products and residues, including grass whey, de-FOS whey and press cake. Using anaerobic digestion, the biogas and biomethane potential of these samples have been analyzed. An analysis of the fertiliser potential of the resulting digestate by-products has also been undertaken. All the feedstocks tested were found to be suitable for biogas production with grass whey, the most suitable candidate with a biogas and biomethane production yield of 895.8 and 544.6 L/kg VS, respectively, followed by de-FOS whey and press cake (597.4/520.3 L/kg VS and 510.7/300.3 L/kg VS, respectively). The results show considerable potential for utilizing biorefinery by-products as a source for renewable energy production, even after several value-added products have been co-produced.