Microbial cellulases have been receiving worldwide attention, as they have enormous potential to process the most abundant cellulosic biomass on this planet and transform it into sustainable biofuels and other value added products. The synergistic action of endoglucanases, exoglucanases, and β-glucosidases is required for the depolymerization of cellulose to fermentable sugars for transformation in to useful products using suitable microorganisms. The lack of a better understanding of the mechanisms of individual cellulases and their synergistic actions is the major hurdles yet to be overcome for large-scale commercial applications of cellulases. We have reviewed various microbial cellulases with a focus on their classification with mechanistic aspects of cellulase hydrolytic action, insights into novel approaches for determining cellulase activity, and potential industrial applications of cellulases.
This study concerned with the optimization of fermentation parameters for the hyper production of mannanase from Fusarium oxysporum SS-25 employing two step statistical strategy and kinetic characterization of crude enzyme preparation. The Plackett-Burman design was first used to screen out the important factors in the culture medium which were found to be: 20% (w/w) wheat bran, 2% (w/w) each of potato peels, soybean meal, malt extract, 1% tryptone, 0.14% NH 4 SO 4, 0.2% KH 2 PO 4 , 0.0002% ZnSO 4 , 0.0005% FeSO 4, 0.01% MnSO 4 , 0.012% SDS, 0.03% NH 4 Cl, 0.1% NaNO 3 in brewer's spent grain based medium with 50% moisture content, inoculated with 2.8×10 higher tear index and 1% increase in brightness when used to treat the wheat straw based kraft pulp. The hydrolytic potential of enzyme was demonstrated on both locust bean gum and guar gum.
This study concerned with the optimization of fermentation parameters for the hyper production of mannanase from Fusarium oxysporum SS-25 employing two step statistical strategy and kinetic characterization of crude enzyme preparation. The Plackett-Burman design was first used to screen out the important factors in the culture medium which were found to be: 20% (w/w) wheat bran, 2% (w/w) each of potato peels, soybean meal, malt extract, 1% tryptone, 0.14% NH 4 SO 4, 0.2% KH 2 PO 4 , 0.0002% ZnSO 4 , 0.0005% FeSO 4, 0.01% MnSO 4 , 0.012% SDS, 0.03% NH 4 Cl, 0.1% NaNO 3 in brewer's spent grain based medium with 50% moisture content, inoculated with 2.8×10 higher tear index and 1% increase in brightness when used to treat the wheat straw based kraft pulp. The hydrolytic potential of enzyme was demonstrated on both locust bean gum and guar gum.
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