BackgroundNo vaccine is currently available for dengue virus (DENV), therefore control programmes usually focus on managing mosquito vector populations. Entomological surveys provide the most common means of characterising vector populations and predicting the risk of local dengue virus transmission. Despite Indonesia being a country strongly affected by DENV, only limited information is available on the local factors affecting DENV transmission and the suitability of available survey methods for assessing risk.Methodology/principal findingsWe conducted entomological surveys in the Banyumas Regency (Central Java) where dengue cases occur on an annual basis. Four villages were sampled during the dry and rainy seasons: two villages where dengue was endemic, one where dengue cases occurred sporadically and one which was dengue-free. In addition to data for conventional larvae indices, we collected data on pupae indices, and collected adult mosquitoes for species identification in order to determine mosquito species composition and population density. Traditionally used larval indices (House indices, Container indices and Breteau indices) were found to be inadequate as indicators for DENV transmission risk. In contrast, species composition of adult mosquitoes revealed that competent vector species were dominant in dengue endemic and sporadic villages.Conclusions/significanceOur data suggested that the utility of traditional larvae indices, which continue to be used in many dengue endemic countries, should be re-evaluated locally. The results highlight the need for validation of risk indicators and control strategies across DENV affected areas here and perhaps elsewhere in SE Asia.

aBstraKMembran permeable banyak dipergunakan dalam penelitian in vitro kultur sel. Kebocoran sel sangat sering terjadi ketika membran permeabel yang dipergunakan memiliki ukuran pori-pori yang relatif besar. Di sisi lain, pemakaian membran permeable dengan pori-pori yang lebih kecil pada studi permabilitas banyak menghadapi kesulitan teknis akibat kecilnya ukuran pori dan densitas pori-pori membran. Penelitian ini menghadirkan teknik memperkecil kebocoran sel pada kultur sel yang mempergunakan membran permabel berpori-pori besar. Vero cell line (CCL-81, ATCC) dipergunakan sebagai sel objek dan dikultur pada membran polyester yang memiliki ukuran pori 3 µm. Visualisasi dengan bantuan mikroskop dipergunakan untuk menganalisa kebocoran sel. Adapun untuk menguji kinerja permeabilitas sel dipergunakan parallel plate flow chamber. Hasil penelitian mendapatkan bahwa temuan teknik yang dipakai mampu menurunkan kebocoran sel secara signifikan pada kultur sel dengan membran permeabel berpori-pori besar. Hasil yang sama juga dijumpai pada pemakaian membran permeabel yang terbuat dari polyester dan polycarbonat.