Abstract. The objective of the study was to characterize the molecular weight of protein in order to be utilized as biological agent of S.litura and their cell or spores production. To investigate the molecular weight of protein was done by SDS-Page electrophoresis. Growth media used for producing B. thuringiensis were T3, LB broth and agricultural waste. The results showed that the molecular weight of protein ranged from 37 to 140 kDa. In DLM and DLKK23 isolates were found ranging from 37 to 40 kDa and from 110 to 130 kDa, respectively. KJ3R5 and KJ3P1 isolates were obtained having three protein bands ranging from 43 to 45, 73 to 80 and 110 to 130 kDa and 45-50, 75-80 and 130-140 kDa, respectively. It was predicted that isolates B. thuringiensis were belonging to Cry IA, Cry IIA, Cry IVC and Cry15c. These crystal proteins were toxic to S. litura. There was no protein bands found in the two last isolates (KJ3R3 and KJ3J4). Production of spore after sporulation in agricultural waste media ranged from 0.5 to 10 6 -2.67 x 10 7 spores/ml showing medium level of toxicity to S. litura.
Kacang panjang termasuk dalam Famili Fabaceae dan merupakan salah satu komoditi sayuran yang banyak diusahakan di daerah dataran rendah pada ketinggian 0-200 m dpl. Permintaan kacang panjang dipasaram terus meningkat, namun pasokan kacang panjang terbatas. Peningkatan produksi kacang panjang dapat dilakukan dengan cara perbaikan tingkat kerapatan tanam. Untuk meningkatkan hasil polong kacang panjang, bisa dilakukan beberapa upaya seperti dengan pengendalian kultur teknis. Bertujuan untuk mengetahui intensitas serangan hama dan penyakit dan perbedaan pengaruh pengendalian kultur teknis terhadap dua lahan tanaman kacang panjang. Penelitian dilaksanakan di lahan kacang panjang milik petani di Kecamatan Lempuing, Kabupaten Ogan Komering Ilir, Provinsi Sumatera Selatan Penelitian ini dilakukan dengan cara pengamatan langsung (Scan sampling) persentase serangan hama dan penyakit pada pertanaman kacang panjang. Pengambilan sampel pada praktek lapangan ini diawali dengan menentukan jumlah guludan yang akan diamati. Penentuan skor gejala serangan hama maupun intensitas penyakit di lapangan ditentukan dengan mengamati gejala masing masing serangan hama dan juga penyakit pada tanaman kacang panjang. Secara keseluruhan intensitas serangan hama dan penyakit pada lahan kacang panjang 1 milik Bapak Johari lebih banyak dibandingkan dengan lahan 2 milik Bapak Sutekno. Ada beberapa faktor yang mungkin mempengaruhi hal tersebut diantaranya tanaman rotasi yang digunakan, umur tanaman, penggunaan fungisida karena lahan 1 tidak menggunakan fungisida sama sekali sedangkan lahan 2 milik Bapak Sutekno mengaplikasikan fungisida pada tanaman kacang panjang miliknya. Kata kunci : tanaman kacang panjang, hama dan penyakit.
Abstract.Bacillus thuringiensis is a gram-positive bacterium that produces crystal proteins toxic (ᴕ-endotoxin) specific to the target insect, but is not toxic to humans and non-target organisms. This study aims to explore the origin of the soil bacterium B. thuringiensis subdistrict Sekayu, Banyuasin, South Sumatra and toxicity to larvae of lepidoptera. Fifty soil samples were taken from Musi Banyuasin District, namely 15 from Kayuare strip 2, 20 from Kayuare and 15 from Lumpatan. Isolation, characterization, identification and screening test were conducted in the laboratorium of Pest and Disease, Agricultural Faculty, Sriwijaya University. Isolat codes were given based on the area origin of the samples. Results of the study showed that from 50 isolates of bacteria that had been isolated, there were 15 bacterial isolates, characterized by morphology and physiology the same as B. thuringiensis, which has round colonies, white, wrinkled edges, slippery, elevation arise, aerobic and gram-positive. Of the 15 codes that contain positive isolates of B. thuringiensis, we have obtained several isolates of the following codes: KJ
There are many types of vegetables that are nutritious for health and metabolism, including cucumbers. Cucumbers are included in the vegetable category because cucumbers are often used as fresh vegetables or pickles. Cucumber cultivation is inseparable from pests and diseases. Pest management efforts must be carried out based on consideration of ecosystem stability. One of the techniques for controlling pests in cucumber plants can be done with technical culture. The research practice aims to determine the effect of technical culture on the diversity of pest types, population and intensity of cucumber pest attacks on two different fields in Tanjung Seteko Village, Indralaya. The research was conducted with a sampling method to determine the population, intensity and pest attack. The research was carried out on two different fields of age and variety. Data analysis was performed using the t test at the 5% confidence level. Pests found in cucumber fields, namely, Aulacophora sp., Succinea sp., Valanga sp., And Leptoglossus sp. Based on observations in the field, technical culture in the village of Tanjung Seteko has a significant effect on the population and intensity of the attack of Succinea sp., Valanga sp. and Leptoglossus being Aulachopora sp. no significant effect.
Propagation of B. thuringiensis in agricultural liquid waste media can be done because the waste still contains nutritional components which contain carbon and nitrogen. The aim of research was to study B. thuringiensis in a liquid formulation with high toxicity against Oryctes larvae. Parameters of observation were spore density, larval mortality and symptoms of both infection or death larvae. Experimental was designed as a completely randomized design with 6 treatments and 4 replications. Oryctes larvae used were 3rd-instar. The treatments were 1). Tofu liquid waste; 2) Tofu Liquid Waste + Bio-urine (1:2); 3) Tofu Liquid Waste + Bio-urine (1:1); 4) Tofu Liquid Waste + Bio-urine (2:1); 5) Bio-urine and 6) NB media (as control). The results showed the highest spore density was found in tofu liquid waste propagation media and biourine (1:2). The highest larval mortality was shown in treatment of Tofu Liquid Waste + Bio-urine (1:2), which was 91.67%. Observations on the symptoms of infection and death in tested larvae were indicated by changes in color of integument and fragility of the larva's body. Bio-insecticide with active ingredient of B. thuringiensis in liquid formulation has potency to be used as biological agent for controlling O. rhinoceros larvae.
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