A novel donor–acceptor–donor (D-A-D) type compound containing pyrazine as the acceptor and triphenylamine as the donor has been designed and synthesized. The photophysical properties and biocompatibility of this probe, namely (OMeTPA)2-Pyr for live cell imaging were systematically investigated, with observed large Stokes shifts, high photostability, and low cytotoxicity. Furthermore, we demonstrated that (OMeTPA)2-Pyr could permeate live cell membranes for labeling. The proposed mechanism of this probe was the binding and shafting through membrane integral transport proteins by electrostatic and hydrophobic interactions. These salient and novel findings can facilitate the strategic design of new pyrazine-fused charge-neutral molecular platforms as fluorescent probes, for long-term in situ dynamic monitoring in live cells.
The metaverse is the integration of multiple new technologies which generates next generation of Internet applications and the state of social, its application in the field of education has the potential to bring the third way for human beings to comprehend the complexity of educational system and the regularities of development of education. This study uses CiteSpace visualization software for literature analysis, investigates critical nodes of current researches related to Edumetaverse, and bases on six core technologies of Edu-metaverse, Edu-metaverse application scenarios are constructed in five dimensions: teaching mode, teaching environment, teaching resources, teaching activities and teaching evaluation. On this basis, it is initially applied to the teaching of a professional course at University H. The results of the accompanying tests of the course are recorded, and the mechanism of action is verified through performance analysis.
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