The new chromophoric reagent, 4.dimethylaminoazobenzene-4'-sulfonyl chloride (dabsyl chloride) was synthesized by reaction of sodium 4-dimethylaminoazobenzene 4'-sulfonate with phosphorus pentachloride. Dabsyl chloride reacted rapidly with all amino acids. aliphatic amines, and polyarnines to form the corresponding dabsyl derivatives. The dabsyl amino acids (lO-11 mol) were visualized on thin layer plates and found to be photo-stable.During the last 12 years. in combination with HPLC, this newly developed labeling reagent has been shown to be very promising for microdetermination of amino acids. aliphatic amines and polyamines, Recently, another new chromophoric reagent. dabsylhydrazine, was synthesized from the reaction of dabsyl chloride with hydrazine. This reagent reacted readily with monosaccharides and carbonyl compounds to form the corresponding chromophoric dabsylhydrazones with strong absorbance at 425 nm. The application of this new reagent in HPLC analysis of monosaccharides was discribed. By use of Nova-PAK CIS reverse-phase column and a concave gradient system of water and acetonitrile as eluent, the detection limits of monosaccharides in the range of 10 pmol have been reached.
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