Downy mildew (Plasmopara viticola) is a significant problem in grape vineyards throughout the growing season. Control of downy mildew is carried out with a combination of host tolerance and chemical applications. Even in vineyards planted with very tolerant varieties (e.g., Concord), control is important in years with ideal pathogen conditions. Fungicides with a single mode of action possess a very high potential for the development of resistance. Resistance has been observed often in the Quinone outside inhibitor (QoI) fungicides, such as strobilurins. We ascertained the levels of QoI resistance in downy mildew colonies on diseased leaves using CAPS-PCR to detect the glycine to alanine mutation (G143A) known to confer a qualitative level of resistance in fungal pathogens. Our data uncovered a small percentage of samples that contain G143A, suggesting an overall low level of QoI resistance. The low prevalence of the resistant single nucleotide polymorphism (SNP) suggests that QoI fungicides should remain a viable control mechanism in Lake Erie vineyards. Additionally, it appears that a viticultural region where tolerant hosts predominant and QoI use is minimal, resistance buildup in the pathogen population will be minimal. Accepted for publication 15 January 2013. Published 22 April 2013.
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