Phytosterols were analyzed in 34 different quinoa accessions,
which
were obtained from the same field trial. Twenty different sterols
were detected, and 17 could be structurally assigned by means of gas
chromatography with mass spectrometry. Sterols were quantitated in
selected ion monitoring mode (GC/MS-SIM) with the novel internal standard
3-O-tert-butyldimethylsilyl-cholestanol
(cholestanyl-TBDMS). GC/MS-SIM response factors of minor sterols were
determined after enrichment by countercurrent chromatography. The
total sterol contents varied from 120 to 180 mg/100 g of seeds, which
is higher than has been described in quinoa before. This was due to
the fact that Δ7-sterols (e.g., Δ7-sitosterol, spinasterol,
and Δ7-avenasterol) were quantitated for the first time in quinoa
and contributed ∼64% to the total sterol content. Clustering
allowed distributing of the 34 different quinoa accessions into four
distinct groups on the basis of the different sterol patterns.
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