Common bean is the most cultivated grain legume consumed globally. It is enriched in proteins, vitamins, minerals and fiber. Anthracnose disease incited by hemibiotrophic fungi Colletotrichum lindemuthianum is the most destructive disease of common beans. The present study was carried out to evaluate 87 common bean genotypes collected from different locations against C. lindemuthianum. Screening of genotypes for anthracnose resistance aids in the identification of 22 highly resistant genotypes namely PL 1, EC-400397, Hur 137, IC-199277, IC-258273, S 2, EC-400442, KB 4, Utkarsh, Hur 15, IC-260299, PDR 14, VL 125, Amber, Arun, EC-398591, EC-121013, S 6, BR 31, IC- 328372, KB 12 and KB 6. These genotypes can act as potential donors of the resistant genes in markerassisted selection (MAS) programmes for transferring the anthracnose resistance gene(s) into the susceptible genotypes.
Background: Vascular wilt caused by Fusarium oxysporum f.sp. lentis Vasu. and Srini. is a serious disease of lentil (Lens culinaris Medikus), causes severe yield losses worldwide. For effective disease resistance breeding the inheritance and mapping of wilt resistance gene (s) is necessary. Therefore, the present investigation was focused on study the mode of inheritance and tag/map gene (s) for fusarium wilt resistance in lentil.
Methods: Bulked segregant analysis (BSA) approach was used to identify markers that were tightly linked to Fusarium wilt resistance gene. The inheritance and mapping of wilt-resistance gene (s) in lentil was investigated in F2 and F2:3 populations derived from L9-12×ILL10965 cross, whereas L9-12 and ILL10965 were susceptible and resistant parents, respectively.
Result: More than two hundreds SSRs markers were surveyed for the parental polymorphism, of which twenty nine were found polymorphic. These polymorphic SSRs were used for the bulked-segregant analysis (BSA) using both parents and its respective resistant and susceptible bulks, and three SSRs viz. PBALC233, PBALC1409 and PBALC203 could distinguish the respective bulks. Linkage analysis showed two SSR markers, PBALC203 and PBALC1409 flanking the wilt resistance gene at 8.2 cM and 9.4 cM distance, respectively. Further, PBLAC233 was also found present on the same linkage group at a distance of 10.2 cM from PBLAC1409.
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